Mammalian ciliary-body epithelial cells in culture produce transforming growth factor-beta

Horst Helbig, Karen L. Kittredge, Miguel Coca-Prados, Janet Davis, Alan G. Palestine, Robert B. Nussenblatt

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Abstract

Transforming growth factor-beta (TGF-beta) has recently been found in the aqueous humor. The present study was initiated to test whether the ciliary-body epithelium, the site of aqueous humor formation, is capable of producing TGF-beta. Human, rat and bovine ciliary epithelial cells were grown in tissue culture and their supernatants were tested for TGF-beta using a bioassay. After acid activation of the samples, TGF-beta activity was found in the supernatants of all three mammalian ciliary epithelial cells. Most of this activity could be blocked by a neutralizing antibody against TGF-beta type 2. Native supernatants did not contain detectable TGF-beta activity. Thus, the ciliary epithelium is capable of producing the inactive form of TGF-beta (mainly type 2) and may be a source of TGF-beta in the aqueous humor.

Original languageEnglish (US)
Pages (from-to)84-87
Number of pages4
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume229
Issue number1
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

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ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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