Malignant transformation by a mutant of the IFN-inducible dsRNA-dependent protein kinase

A. E. Koromilas, S. Roy, Glen N Barber, M. G. Katze, N. Sonenberg

Research output: Contribution to journalArticle

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Abstract

The double-stranded RNA-dependent protein kinase (dsRNA-PK) is thought to be a key mediator of the antiviral and antiproliferative effects of interferons (IFNs). Studies examining the physiological function of the kinase suggest that it participates in cell growth and differentiation by regulating protein synthesis. Autophosphorylation and consequent activation of dsRNA-PK in vitro and in vivo result in phosphorylation of the α subunit of eukaryotic initiation factor-2 (eIF-2) and inhibition of protein synthesis. Expression of a functionally defective mutant of human dsRNA-PK in NIH 3T3 cells resulted in malignant transformation, suggesting that dsRNA-PK may function as a suppressor of cell proliferation and tumorigenesis.

Original languageEnglish
Pages (from-to)1685-1689
Number of pages5
JournalScience
Volume257
Issue number5077
StatePublished - Jan 1 1992
Externally publishedYes

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eIF-2 Kinase
Double-Stranded RNA
Protein Kinases
Interferons
Eukaryotic Initiation Factor-2
NIH 3T3 Cells
Antiviral Agents
Cell Differentiation
Carcinogenesis
Proteins
Phosphotransferases
Phosphorylation
Cell Proliferation
Growth

ASJC Scopus subject areas

  • General

Cite this

Koromilas, A. E., Roy, S., Barber, G. N., Katze, M. G., & Sonenberg, N. (1992). Malignant transformation by a mutant of the IFN-inducible dsRNA-dependent protein kinase. Science, 257(5077), 1685-1689.

Malignant transformation by a mutant of the IFN-inducible dsRNA-dependent protein kinase. / Koromilas, A. E.; Roy, S.; Barber, Glen N; Katze, M. G.; Sonenberg, N.

In: Science, Vol. 257, No. 5077, 01.01.1992, p. 1685-1689.

Research output: Contribution to journalArticle

Koromilas, AE, Roy, S, Barber, GN, Katze, MG & Sonenberg, N 1992, 'Malignant transformation by a mutant of the IFN-inducible dsRNA-dependent protein kinase', Science, vol. 257, no. 5077, pp. 1685-1689.
Koromilas AE, Roy S, Barber GN, Katze MG, Sonenberg N. Malignant transformation by a mutant of the IFN-inducible dsRNA-dependent protein kinase. Science. 1992 Jan 1;257(5077):1685-1689.
Koromilas, A. E. ; Roy, S. ; Barber, Glen N ; Katze, M. G. ; Sonenberg, N. / Malignant transformation by a mutant of the IFN-inducible dsRNA-dependent protein kinase. In: Science. 1992 ; Vol. 257, No. 5077. pp. 1685-1689.
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