Major histocompatibility complex class I molecules of nonhuman primates

David I. Watkins, Mari Kannagi, Marc E. Stone, Norman L. Letvin

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

The usefulness of nonhuman primates in immunologically relevant research has until now been limited by difficulties in characterizing the major histocompatibility (MHC) gene products of these species. We have now biochemically characterized the MHC-encoded class I molecules from four different species of nonhuman primates using antibodies directed against human MHC class I structures and one-dimensional isoelectric focusing (1-D IEF). We demonstrated the functional relevancy of this technique of MHC typing by generating virus-specific cytotoxic T cells and assaying their cytotoxic activity against a panel of virus-transformed cells that expressed the same or differing class I structures. Only virus-infected cell lines expressing MHC class I antigens identical to those of the cytotoxic T lymphocyte population were lysed. This simple method of MHC class I typing using 1-D IEF will be useful in immunological research involving nonhuman primates and in nonhuman primate colony management.

Original languageEnglish (US)
Pages (from-to)1425-1432
Number of pages8
JournalEuropean Journal of Immunology
Volume18
Issue number9
DOIs
StatePublished - Sep 1988
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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