Magnetically labeled insulin-secreting cells

Jose Oca-Cossio, Hui Mao, Nata Khokhlova, Christopher Mc E. Kennedy, Jonathan W. Kennedy, Cheryl L. Stabler, Encai Hao, Athanassios Sambanis, Nicholas E. Simpson, Ioannis Constantinidis

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Iron oxide nanoparticles have been shown to magnetically label cells in order to visualize them in vivo via MR imaging. This technology has yet to be implemented in insulin secreting cells, thus it is not known whether the presence of these nanoparticles in the cytoplasm of the cells affects insulin secretion. This study investigates the effectiveness and consequence of labeling mouse insulinoma βTC3 and βTC-tet cells with monocrystalline iron oxide nanoparticles (MION). Our data show that MION can be internalized in both βTC3 and βTC-tet cells following a 24h exposure to 0.02mg/ml MION solution. The metabolic and secretory activities of both MION-labeled cell lines were statistically indistinguishable from sham treatment. Furthermore, cell viability and apoptosis remained constant throughout the cell's exposure to MION. Finally, MR images demonstrated significant contrast between labeled and sham-treated cells. Thus, labeling murine insulinoma cell lines with magnetic iron oxide nanoparticles does not hinder their insulin secretion, while it provides MR imaging contrast.

Original languageEnglish (US)
Pages (from-to)569-575
Number of pages7
JournalBiochemical and biophysical research communications
Volume319
Issue number2
DOIs
StatePublished - Jun 25 2004

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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