Abstract
Destruction of striatal neurons in the rat brain, induced by intracerebral injection of N-methyl D-aspartic acid (NMDA), has been visualized noninvasively by magnetic resonance imaging (MRI). The changes in images were monitored from 12 h to one month after the stereotaxic microinfusion of NMDA (10 μg in 0.4 μl) into the striatum, using a T2- weighted rapid acquisition by relaxation enhancement (RARE) sequence. A localised hyperintense (bright) area was visible after 12 h at the site of the injection, and it persisted for the next three days. The size of the hyperintense area decreased thereafter and, after one week, the increased brightness was restricted to the lateral ventricle. Post-mortem histological examination, done after one month, showed a dilated lateral ventricle. The size and location of the lesioned area, identified in histological sections, corresponded to the hyperintense area observed during these initial days after NMDA lesion. The present study demonstrates that noninvasive MRI techniques, using a typical RARE sequence, offer a powerful tool for the early detection of neurotoxic lesion of the brain area, although some caution is required in its use for estimating the size of the lesioned area three days after its formation. The present findings indicate that, in long-term studies, alterations of the neighbouring structures, such as enlargement of the ventricular system, may confound the MRI evaluation of neurotoxic lesions in vive.
| Original language | English |
|---|---|
| Pages (from-to) | 273-277 |
| Number of pages | 5 |
| Journal | Brain Research Bulletin |
| Volume | 40 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1996 |
| Externally published | Yes |
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Keywords
- MRI
- NMDA lesion
- RARE sequence, Gliosis
- Rat
- Striatum
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Magnetic resonance imaging of temporal changes of neurotoxic lesion in the rat. / John, Joshi; Govind, Varan; Raghunathan, Partha; Kumar, Velayudhan Mohan.
In: Brain Research Bulletin, Vol. 40, No. 4, 1996, p. 273-277.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Magnetic resonance imaging of temporal changes of neurotoxic lesion in the rat
AU - John, Joshi
AU - Govind, Varan
AU - Raghunathan, Partha
AU - Kumar, Velayudhan Mohan
PY - 1996
Y1 - 1996
N2 - Destruction of striatal neurons in the rat brain, induced by intracerebral injection of N-methyl D-aspartic acid (NMDA), has been visualized noninvasively by magnetic resonance imaging (MRI). The changes in images were monitored from 12 h to one month after the stereotaxic microinfusion of NMDA (10 μg in 0.4 μl) into the striatum, using a T2- weighted rapid acquisition by relaxation enhancement (RARE) sequence. A localised hyperintense (bright) area was visible after 12 h at the site of the injection, and it persisted for the next three days. The size of the hyperintense area decreased thereafter and, after one week, the increased brightness was restricted to the lateral ventricle. Post-mortem histological examination, done after one month, showed a dilated lateral ventricle. The size and location of the lesioned area, identified in histological sections, corresponded to the hyperintense area observed during these initial days after NMDA lesion. The present study demonstrates that noninvasive MRI techniques, using a typical RARE sequence, offer a powerful tool for the early detection of neurotoxic lesion of the brain area, although some caution is required in its use for estimating the size of the lesioned area three days after its formation. The present findings indicate that, in long-term studies, alterations of the neighbouring structures, such as enlargement of the ventricular system, may confound the MRI evaluation of neurotoxic lesions in vive.
AB - Destruction of striatal neurons in the rat brain, induced by intracerebral injection of N-methyl D-aspartic acid (NMDA), has been visualized noninvasively by magnetic resonance imaging (MRI). The changes in images were monitored from 12 h to one month after the stereotaxic microinfusion of NMDA (10 μg in 0.4 μl) into the striatum, using a T2- weighted rapid acquisition by relaxation enhancement (RARE) sequence. A localised hyperintense (bright) area was visible after 12 h at the site of the injection, and it persisted for the next three days. The size of the hyperintense area decreased thereafter and, after one week, the increased brightness was restricted to the lateral ventricle. Post-mortem histological examination, done after one month, showed a dilated lateral ventricle. The size and location of the lesioned area, identified in histological sections, corresponded to the hyperintense area observed during these initial days after NMDA lesion. The present study demonstrates that noninvasive MRI techniques, using a typical RARE sequence, offer a powerful tool for the early detection of neurotoxic lesion of the brain area, although some caution is required in its use for estimating the size of the lesioned area three days after its formation. The present findings indicate that, in long-term studies, alterations of the neighbouring structures, such as enlargement of the ventricular system, may confound the MRI evaluation of neurotoxic lesions in vive.
KW - MRI
KW - NMDA lesion
KW - RARE sequence, Gliosis
KW - Rat
KW - Striatum
UR - http://www.scopus.com/inward/record.url?scp=2442660087&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2442660087&partnerID=8YFLogxK
U2 - 10.1016/0361-9230(96)00047-0
DO - 10.1016/0361-9230(96)00047-0
M3 - Article
C2 - 8842412
AN - SCOPUS:2442660087
VL - 40
SP - 273
EP - 277
JO - Brain Research Bulletin
JF - Brain Research Bulletin
SN - 0361-9230
IS - 4
ER -