Macrophages archive HIV-1 virions for dissemination in trans

Natalia Sharova, Catherine Swingler, Mark Sharkey, Mario Stevenson

Research output: Contribution to journalArticle

143 Scopus citations

Abstract

Viruses have evolved various strategies in order to persist within the host. To date, most information on mechanisms of HIV-1 persistence has been derived from studies with lymphocytes, but there is little information regarding mechanisms that govern HIV-1 persistence in macrophages. It has previously been demonstrated that virus assembly in macrophages occurs in cytoplasmic vesicles, which exhibit the characteristics of multivesicular bodies or late endosomes. The infectious stability of virions that assemble intracellularly in macrophages has not been evaluated. We demonstrate that virions assembling intracellularly in primary macrophages retain infectivity for extended intervals. Infectious virus was recovered directly from cytoplasmic lysates of macrophages and could be transmitted from macrophages to peripheral blood lymphocytes in trans 6 weeks after ongoing viral replication was blocked. Cell-associated virus decayed significantly from 1 to 2 weeks post infection, but decreased minimally thereafter. The persistence of intracellular virions did not require the viral accessory proteins Vpu or Nef. The stable sequestration of infectious virions within cytoplasmic compartments of macrophages may represent an additional mechanism for viral persistence in HIV-1-infected individuals.

Original languageEnglish (US)
Pages (from-to)2481-2489
Number of pages9
JournalEMBO Journal
Volume24
Issue number13
DOIs
StatePublished - Jul 6 2005
Externally publishedYes

Keywords

  • Cytoplasmic virions
  • HIV-1
  • Macrophages
  • Viral persistence

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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