Macrophage inhibitory factor-A3 derived from Mycobacterium avium serovar 2 inhibits candidacidal activity of murine peritoneal macrophages

Murray E. Hines, Carolyn Cray, François Elvinger, Norman H. Altman

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Macrophage inhibitory factor-A3 (MIF-A3), a fraction derived from Mycobacterium avium serovar 2 inhibited candidacidal activity in macrophages from C57BL/6, C57BL/10, C3H/HeJ and A/J strains of mice. Inhibition of candidacidal activity was demonstrated at MIF-A3 concentrations ranging from 100-400 μg/ml in macrophages without additional stimulators (exception C3H/HeJ macrophages) and in macrophages additionally stimulated with 200 U/ml interferon-γ, 100 ng/ml phorbol myristate acetate and 0.4 ng/ml E. coli lipopolysaccharide from all mouse strains tested. The decreased candidacidal effect produced by MIF-A3 was dose-dependent and appeared greatest in macrophages treated with phorbol myristate acetate and lipopolysaccharide. This effect was neutralized by the addition of goat anti-MIF-A3 antiserum. Macrophages from the Bcg(s) mouse strains (C57BL/6 and C57BL/10) were more sensitive to the effect(s) of MIF-A3 than macrophages from the Bcg(r) mouse strains (C3H/HeJ and A/J).

Original languageEnglish (US)
Pages (from-to)295-302
Number of pages8
JournalVeterinary Microbiology
Issue number3-4
StatePublished - Dec 1 1996



  • Anti-MIF-3 antiserum
  • Candida albicans
  • Macrophage inhibition
  • Macrophage inhibitory factor-A3 (MIF-A3)
  • Murine peritoneal macrophages
  • Mycobacterium avium serovar 2

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

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