Macrophage-derived chemokine expression in classical Hodgkin's lymphoma

Application of tissue microarrays

Cyrus V. Hedvat, Elaine S. Jaffe, Jing Qin, Daniel A. Filippa, Carlos Cordon-Cardo, Giovanna Tosato, Stephen D Nimer, Julie Teruya-Feldstein

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Hodgkin's disease (HD) is a lymphoid malignancy characterized by the presence of Reed-Sternberg (RS) and Hodgkin's cells in a background of mixed inflammatory cells and stromal reaction. Studies have documented that HD is a neoplasm associated with abnormal cytokine and chemokine production. To define the expression of macrophagederived chemokine (MDC) in HD, 57 cases (18 lymphocyte predominant, 11 mixed cellularity, 28 nodular sclerosis) were stained for MDC by immunohistochemistry and compared with reactive lymph nodes as controls. MDC was expressed by RS cells in classical HD (CHD) and showed a distinct cytoplasmic and Golgi localization. Accumulating evidence suggests that lymphocyte-predominant HD (LPHD) represents an entity distinct from CHD, with different biological properties and clinical course. On the basis of the high level of MDC staining alone, CHD could be distinguished from LPHD (P < .001), which showed only faint staining of scattered histiocytes similar to control tissues. CHD cases with high MDC mRNA levels showed high levels of MDC protein expression by immunohistochemistry (P < .001) and significant eosinophil infiltration, suggesting that MDC may represent another molecule that plays a critical role in eosinophil recruitment. We also analyzed 102 cases of non-Hodgkin's lymphoma and normal spleen, lymph node, and thymic tissue. High levels of MDC expression were specific to CHD cases because only low levels of MDC were observed in a minor subset of LPHD, NHL or normal lymphoid tissues.

Original languageEnglish
Pages (from-to)1270-1276
Number of pages7
JournalModern Pathology
Volume14
Issue number12
DOIs
StatePublished - Dec 31 2001
Externally publishedYes

Fingerprint

Chemokine CCL22
Hodgkin Disease
Chemokines
Reed-Sternberg Cells
Lymphocytes
Eosinophils
Lymph Nodes
Immunohistochemistry
Staining and Labeling
Histiocytes
Lymphocyte Subsets
Sclerosis
Lymphoid Tissue
Stromal Cells
Non-Hodgkin's Lymphoma
Neoplasms

Keywords

  • Chemokine
  • Hodgkin's lymphoma
  • Lymphoma
  • MDC
  • Tissue microarray

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Hedvat, C. V., Jaffe, E. S., Qin, J., Filippa, D. A., Cordon-Cardo, C., Tosato, G., ... Teruya-Feldstein, J. (2001). Macrophage-derived chemokine expression in classical Hodgkin's lymphoma: Application of tissue microarrays. Modern Pathology, 14(12), 1270-1276. https://doi.org/10.1038/modpathol.3880473

Macrophage-derived chemokine expression in classical Hodgkin's lymphoma : Application of tissue microarrays. / Hedvat, Cyrus V.; Jaffe, Elaine S.; Qin, Jing; Filippa, Daniel A.; Cordon-Cardo, Carlos; Tosato, Giovanna; Nimer, Stephen D; Teruya-Feldstein, Julie.

In: Modern Pathology, Vol. 14, No. 12, 31.12.2001, p. 1270-1276.

Research output: Contribution to journalArticle

Hedvat, CV, Jaffe, ES, Qin, J, Filippa, DA, Cordon-Cardo, C, Tosato, G, Nimer, SD & Teruya-Feldstein, J 2001, 'Macrophage-derived chemokine expression in classical Hodgkin's lymphoma: Application of tissue microarrays', Modern Pathology, vol. 14, no. 12, pp. 1270-1276. https://doi.org/10.1038/modpathol.3880473
Hedvat, Cyrus V. ; Jaffe, Elaine S. ; Qin, Jing ; Filippa, Daniel A. ; Cordon-Cardo, Carlos ; Tosato, Giovanna ; Nimer, Stephen D ; Teruya-Feldstein, Julie. / Macrophage-derived chemokine expression in classical Hodgkin's lymphoma : Application of tissue microarrays. In: Modern Pathology. 2001 ; Vol. 14, No. 12. pp. 1270-1276.
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