Lysophosphatidic acid promotes survival and differentiation of rat Schwann cells

Yiwen Li, Marco I. Gonzalez, Judy L. Meinkoth, Jeffrey Field, Marcelo G. Kazanietz, Gihan I. Tennekoon

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Lysophosphatidic acid (LPA; 1-acyl-sn-glycerol-3-phosphate), an abundant constituent of serum, mediates multiple biological responses via G protein-coupled serpentine receptors. Schwann cells express the LPA receptors (Edg receptors), which, once activated, have the potential to signal through Gαi to activate p21ras and phosphatidylinositol 3-kinase, through Gαq to activate phospholipase C, or through Gq12/13 to activate the Rho pathway. We found that the addition of serum or LPA to serum-starved Schwann cells rapidly (10 min) induced the appearance of actin stress fibers via a Rho-mediated pathway. Furthermore, LPA was able to rescue Schwann cells from apoptosis in a Gαi/phosphatidyl-inositol 3-kinase/MEK/MAPK-dependent manner. In addition, LPA increased the expression of myelin protein P0 in Schwann cells in a Gαi-independent manner but dependent on protein kinase C. By means of pharmacological and overexpression approaches, we found that the novel isozyme protein kinase Cδ was required for myelin P0 expression. Thus, the multiple effects of LPA in Schwann cells (actin reorganization, survival, and myelin gene expression) appear to be mediated through the different G protein-dependent pathways activated by the LPA receptor.

Original languageEnglish
Pages (from-to)9585-9591
Number of pages7
JournalJournal of Biological Chemistry
Volume278
Issue number11
DOIs
StatePublished - Mar 14 2003
Externally publishedYes

Fingerprint

Schwann Cells
Rats
Cells
Lysophosphatidic Acid Receptors
Myelin Sheath
GTP-Binding Proteins
Protein Kinase C
Actins
MAP Kinase Kinase Kinase 3
Myelin P0 Protein
Serum
Phosphatidylinositol 3-Kinase
Lysosphingolipid Receptors
Stress Fibers
Mitogen-Activated Protein Kinase Kinases
Type C Phospholipases
G-Protein-Coupled Receptors
Phosphatidylinositols
Gene expression
Isoenzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Li, Y., Gonzalez, M. I., Meinkoth, J. L., Field, J., Kazanietz, M. G., & Tennekoon, G. I. (2003). Lysophosphatidic acid promotes survival and differentiation of rat Schwann cells. Journal of Biological Chemistry, 278(11), 9585-9591. https://doi.org/10.1074/jbc.M213244200

Lysophosphatidic acid promotes survival and differentiation of rat Schwann cells. / Li, Yiwen; Gonzalez, Marco I.; Meinkoth, Judy L.; Field, Jeffrey; Kazanietz, Marcelo G.; Tennekoon, Gihan I.

In: Journal of Biological Chemistry, Vol. 278, No. 11, 14.03.2003, p. 9585-9591.

Research output: Contribution to journalArticle

Li, Y, Gonzalez, MI, Meinkoth, JL, Field, J, Kazanietz, MG & Tennekoon, GI 2003, 'Lysophosphatidic acid promotes survival and differentiation of rat Schwann cells', Journal of Biological Chemistry, vol. 278, no. 11, pp. 9585-9591. https://doi.org/10.1074/jbc.M213244200
Li, Yiwen ; Gonzalez, Marco I. ; Meinkoth, Judy L. ; Field, Jeffrey ; Kazanietz, Marcelo G. ; Tennekoon, Gihan I. / Lysophosphatidic acid promotes survival and differentiation of rat Schwann cells. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 11. pp. 9585-9591.
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