Lyar Is a New Ligand for Retinal Pigment Epithelial Phagocytosis

Feiye Guo, Ying Ding, Nora B. Caberoy, Gabriela Alvarado, Robert Liu, Chen Shen, Jisu Yu, Yixiong Zhou, Enrique Salero, Michelle E. Leblanc, Weiwen Wang, Wei Li

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Phagocytosis is critical to tissue homeostasis, as highlighted by phagocytosis defect of retinal pigment epithelial (RPE) cells with debris accumulation, photoreceptor degeneration and blindness. Phagocytosis ligands are the key to delineating molecular mechanisms and functional roles of phagocytes, but are traditionally identified in individual cases with technical challenges. We recently developed open reading frame phage display (OPD) for phagocytosis-based functional cloning (PFC) to identify unknown ligands. One of the identified ligands was Ly-1 antibody reactive clone (Lyar) with functions poorly defined. Herein, we characterized Lyar as a new ligand to stimulate RPE phagocytosis. In contrast to its reported nucleolar expression, immunohistochemistry showed that Lyar was highly expressed in photoreceptor outer segments (POSs) of the retina. Cytoplasmic Lyar was released from apoptotic cells, and selectively bound to shed POSs and apoptotic cells, but not healthy cells. POS vesicles engulfed through Lyar-dependent pathway were targeted to phagosomes and colocalized with phagosome marker Rab7. These results suggest that Lyar is a genuine RPE phagocytosis ligand, which in turn supports the validity of OPD/PFC as the only available approach for unbiased identification of phagocytosis ligands with broad applicability to various phagocytes. J. Cell. Biochem. 116: 2177-2187, 2015.

Original languageEnglish (US)
Pages (from-to)2177-2187
Number of pages11
JournalJournal of cellular biochemistry
Volume116
Issue number10
DOIs
StatePublished - Oct 1 2015

Keywords

  • LY-1 ANTIBODY REACTIVE CLONE
  • LYAR
  • PHAGOCYTOSIS
  • PHAGOCYTOSIS LIGAND
  • RETINAL PIGMENT EPITHELIAL CELL
  • RPE

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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