Luteinizing hormone-releasing hormone (LH-RH) activity of some synthetic polypeptides. I. Fragments shorter than decapeptide

Andrew V Schally, A. Arimura, W. H. Carter, T. W. Redding, R. Geiger, W. König, H. Wissman, G. Jaeger, J. Sandow, N. Yanaihara, C. Yanaihara, T. Hashimoto, M. Sakagami

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Abstract

The luteinizing-releasing hormone (LH-RH) activity of II synthetic polypeptides, some of which were speculatively reported as having LH-RH activity, was assayed in vivo and in vitro against pure natural and synthetic LH-RH. In vivo tests showed that (pyro)Glu-Tyr-Arg-Trp-NH2 had only 1 part in 7800 of the activity of the LH-RH decapeptide. (Pyro) Glu-Val-Ser-NH2, (pyro)Glu-Ser-Val-NH2 and (pyro)Glu-Gln-Ala-NH2, were inactive in vivo in doses as high as 5 - 20 μg/rat. Synthetic (pyro)Glu-His-Pro-amide (thyrotropin-releasing hormone) and a synthetic decapeptide proposed as growth-hormone-releasing hormone showed no LH-RH activity in doses up to 100 μg. N-terminal tripeptide and tetrapeptide fragments of LH-RH as well as the C-terminal octapeptide of LH-RH were also inactive. The C-terminal nonapeptide had an extremely low LH-RH activity (about 1 part in 50,000). The structure-activity relationship of LH-RH has been briefly discussed.

Original languageEnglish
Pages (from-to)366-375
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume48
Issue number2
DOIs
StatePublished - Jul 25 1972
Externally publishedYes

Fingerprint

Gonadotropin-Releasing Hormone
Peptides
Growth Hormone-Releasing Hormone
Thyrotropin-Releasing Hormone
Hormones
Structure-Activity Relationship
Luteinizing Hormone
Rats

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Luteinizing hormone-releasing hormone (LH-RH) activity of some synthetic polypeptides. I. Fragments shorter than decapeptide. / Schally, Andrew V; Arimura, A.; Carter, W. H.; Redding, T. W.; Geiger, R.; König, W.; Wissman, H.; Jaeger, G.; Sandow, J.; Yanaihara, N.; Yanaihara, C.; Hashimoto, T.; Sakagami, M.

In: Biochemical and Biophysical Research Communications, Vol. 48, No. 2, 25.07.1972, p. 366-375.

Research output: Contribution to journalArticle

Schally, AV, Arimura, A, Carter, WH, Redding, TW, Geiger, R, König, W, Wissman, H, Jaeger, G, Sandow, J, Yanaihara, N, Yanaihara, C, Hashimoto, T & Sakagami, M 1972, 'Luteinizing hormone-releasing hormone (LH-RH) activity of some synthetic polypeptides. I. Fragments shorter than decapeptide', Biochemical and Biophysical Research Communications, vol. 48, no. 2, pp. 366-375. https://doi.org/10.1016/S0006-291X(72)80060-3
Schally, Andrew V ; Arimura, A. ; Carter, W. H. ; Redding, T. W. ; Geiger, R. ; König, W. ; Wissman, H. ; Jaeger, G. ; Sandow, J. ; Yanaihara, N. ; Yanaihara, C. ; Hashimoto, T. ; Sakagami, M. / Luteinizing hormone-releasing hormone (LH-RH) activity of some synthetic polypeptides. I. Fragments shorter than decapeptide. In: Biochemical and Biophysical Research Communications. 1972 ; Vol. 48, No. 2. pp. 366-375.
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