Low oxygen stimulates proliferation of fibroblasts seeded as single cells

Vincent Falanga, Robert S. Kirsner

Research output: Contribution to journalArticlepeer-review

77 Scopus citations


In standard tissue culture conditions (20% oxygen), single human dermal fibroblasts (one cell per well) do not proliferate. We now report that low oxygen tension is a potent stimulus for the proliferation and expansion of human adult and neonatal dermal fibroblasts seeded as single cells. This preferential single-cell proliferation in low oxygen is shown to be also a feature of human lung and dermal rodent fibroblasts, but not of human fibrosarcoma and immortalized 3T3 cells, which proliferate without difficulty in standard oxygen conditions. It is suggested that single-cell proliferation and its dramatic stimulation in low oxygen may represent a fundamental biologic process with an opportunity to better understand mammalian cell growth regulation.

Original languageEnglish (US)
Pages (from-to)506-510
Number of pages5
JournalJournal of Cellular Physiology
Issue number3
StatePublished - Mar 1993

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology


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