Low nadir CD4+ T-cell counts predict gut dysbiosis in HIV-1 infection

Yolanda Guillén, Marc Noguera-Julian, Javier Rivera, Maria Casadellà, Alexander S. Zevin, Muntsa Rocafort, Mariona Parera, Cristina Rodríguez, Marçal Arumí, Jorge Carrillo, Beatriz Mothe, Carla Estany, Josep Coll, Isabel Bravo, Cristina Herrero, Jorge Saz, Guillem Sirera, Ariadna Torrella, Jordi Navarro, Manuel CrespoEugènia Negredo, Christian Brander, Julià Blanco, Maria Luz Calle, Nichole Klatt, Bonaventura Clotet, Roger Paredes

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Human immunodeficiency virus (HIV)-1 infection causes severe gut and systemic immune damage, but its effects on the gut microbiome remain unclear. Previous shotgun metagenomic studies in HIV-negative subjects linked low-microbial gene counts (LGC) to gut dysbiosis in diseases featuring intestinal inflammation. Using a similar approach in 156 subjects with different HIV-1 phenotypes, we found a strong, independent, dose–effect association between nadir CD4+ T-cell counts and LGC. As in other diseases involving intestinal inflammation, the gut microbiomes of subjects with LGC were enriched in gram-negative Bacteroides, acetogenic bacteria and Proteobacteria, which are able to metabolize reactive oxygen and nitrogen species; and were depleted in oxygen-sensitive methanogenic archaea and sulfate-reducing bacteria. Interestingly, subjects with LGC also showed increased butyrate levels in direct fecal measurements, consistent with enrichment in Roseburia intestinalis despite reductions in other butyrate producers. The microbiomes of subjects with LGC were also enriched in bacterial virulence factors, as well as in genes associated with beta-lactam, lincosamide, tetracycline, and macrolide resistance. Thus, low nadir CD4+ T-cell counts, rather than HIV-1 serostatus per se, predict the presence of gut dysbiosis in HIV-1 infected subjects. Such dysbiosis does not display obvious HIV-specific features; instead, it shares many similarities with other diseases featuring gut inflammation.

Original languageEnglish (US)
Pages (from-to)232-246
Number of pages15
JournalMucosal Immunology
Volume12
Issue number1
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

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Microbial Genes
Dysbiosis
Virus Diseases
CD4 Lymphocyte Count
HIV-1
T-Lymphocytes
Intestinal Diseases
Butyrates
Inflammation
Lincosamides
HIV
Bacteria
Tetracycline Resistance
Reactive Nitrogen Species
Metagenomics
Proteobacteria
Bacteroides
Microbiota
Archaea
Macrolides

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Guillén, Y., Noguera-Julian, M., Rivera, J., Casadellà, M., Zevin, A. S., Rocafort, M., ... Paredes, R. (2019). Low nadir CD4+ T-cell counts predict gut dysbiosis in HIV-1 infection. Mucosal Immunology, 12(1), 232-246. https://doi.org/10.1038/s41385-018-0083-7

Low nadir CD4+ T-cell counts predict gut dysbiosis in HIV-1 infection. / Guillén, Yolanda; Noguera-Julian, Marc; Rivera, Javier; Casadellà, Maria; Zevin, Alexander S.; Rocafort, Muntsa; Parera, Mariona; Rodríguez, Cristina; Arumí, Marçal; Carrillo, Jorge; Mothe, Beatriz; Estany, Carla; Coll, Josep; Bravo, Isabel; Herrero, Cristina; Saz, Jorge; Sirera, Guillem; Torrella, Ariadna; Navarro, Jordi; Crespo, Manuel; Negredo, Eugènia; Brander, Christian; Blanco, Julià; Calle, Maria Luz; Klatt, Nichole; Clotet, Bonaventura; Paredes, Roger.

In: Mucosal Immunology, Vol. 12, No. 1, 01.01.2019, p. 232-246.

Research output: Contribution to journalArticle

Guillén, Y, Noguera-Julian, M, Rivera, J, Casadellà, M, Zevin, AS, Rocafort, M, Parera, M, Rodríguez, C, Arumí, M, Carrillo, J, Mothe, B, Estany, C, Coll, J, Bravo, I, Herrero, C, Saz, J, Sirera, G, Torrella, A, Navarro, J, Crespo, M, Negredo, E, Brander, C, Blanco, J, Calle, ML, Klatt, N, Clotet, B & Paredes, R 2019, 'Low nadir CD4+ T-cell counts predict gut dysbiosis in HIV-1 infection', Mucosal Immunology, vol. 12, no. 1, pp. 232-246. https://doi.org/10.1038/s41385-018-0083-7
Guillén Y, Noguera-Julian M, Rivera J, Casadellà M, Zevin AS, Rocafort M et al. Low nadir CD4+ T-cell counts predict gut dysbiosis in HIV-1 infection. Mucosal Immunology. 2019 Jan 1;12(1):232-246. https://doi.org/10.1038/s41385-018-0083-7
Guillén, Yolanda ; Noguera-Julian, Marc ; Rivera, Javier ; Casadellà, Maria ; Zevin, Alexander S. ; Rocafort, Muntsa ; Parera, Mariona ; Rodríguez, Cristina ; Arumí, Marçal ; Carrillo, Jorge ; Mothe, Beatriz ; Estany, Carla ; Coll, Josep ; Bravo, Isabel ; Herrero, Cristina ; Saz, Jorge ; Sirera, Guillem ; Torrella, Ariadna ; Navarro, Jordi ; Crespo, Manuel ; Negredo, Eugènia ; Brander, Christian ; Blanco, Julià ; Calle, Maria Luz ; Klatt, Nichole ; Clotet, Bonaventura ; Paredes, Roger. / Low nadir CD4+ T-cell counts predict gut dysbiosis in HIV-1 infection. In: Mucosal Immunology. 2019 ; Vol. 12, No. 1. pp. 232-246.
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