Low magnification confocal microscopy of tumor angiogenesis

George McNamara, Anna Yanai, Vazgen Khankaldyyan, Walter E. Laug, Jeff Boden, Keith A Webster, Yiwen Li, Rong Wen

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Blood vessels are critical to normal mammalian development, tissue repair, and growth and treatment of cancer. Mouse research models enable mechanistic studies of blood vessels. We detail how to perfuse mice with fluorescent tomato lectin or the lipophilic fluorophore DiI. We provide details on how to image fluorescently labeled blood vessels.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages149-175
Number of pages27
Volume1075
ISBN (Print)9781588293510
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1075
ISSN (Print)10643745

Keywords

  • Angiogenesis
  • Blood vessels
  • Confocal microscope
  • DiI
  • Fluorescent lectin

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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