Low frequency of NRAS and KRAS2 gene mutations in childhood myelodysplastic syndromes

Biljana Jekic, Ivana Novakovic, Ljiljana Lukovic, Milos Kuzmanovic, Branka Popovic, Irena Pastar, Jelena Milasin, Gordana Bunjevacki, Vera Bunjevacki

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8 Scopus citations

Abstract

In children, myelodysplastic syndromes (MDS) represent less then 10% of all hematological malignancies; consequently, molecular genetic studies dealing with this group of patients are scarce. We have analyzed 35 archival bone marrow samples of children with MDS for the presence of mutations in the first and second exons of the NRAS and KRAS2 genes. Mutations were detected with single-strand conformation polymorphism analysis in three patients. One patient harbored a mutation in the second exon of NRAS and two patients in the second exon of KRAS2. Sequencing was performed in two samples and novel mutations were found in both. One patient had a missense mutation in codon 45 of NRAS; the other had a silent mutation in codon 53 and a missense mutation in codon 55 of KRAS2.

Original languageEnglish (US)
Pages (from-to)180-182
Number of pages3
JournalCancer Genetics and Cytogenetics
Volume154
Issue number2
DOIs
StatePublished - Oct 15 2004
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Jekic, B., Novakovic, I., Lukovic, L., Kuzmanovic, M., Popovic, B., Pastar, I., Milasin, J., Bunjevacki, G., & Bunjevacki, V. (2004). Low frequency of NRAS and KRAS2 gene mutations in childhood myelodysplastic syndromes. Cancer Genetics and Cytogenetics, 154(2), 180-182. https://doi.org/10.1016/j.cancergencyto.2004.02.025