Loss of Intra-Epithelial Endocervical Gamma Delta (GD) 1 T Cells in HIV-Infected Women

Natasa Strbo; Maria L. Alcaide; Laura Romero; Hector Bolivar; Deborah Jones; Eckhard R. Podack; Margaret A. Fischl

doi:10.1111/aji.12458

Loss of Intra-Epithelial Endocervical Gamma Delta (GD) 1 T Cells in HIV-Infected Women

Natasa Strbo, Maria L. Alcaide, Laura Romero, Hector Bolivar, Deborah Jones, Eckhard R. Podack, Margaret A. Fischl

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Problem: Human gamma delta (GD) T cells play a well-documented role in epithelial barrier surveillance and protection. Two subsets of GD T cells, defined by the use of either the Vdelta2 (GD2) or Vdelta1 (GD1) TCR, predominate. We hypothesized that endocervical GD T cells play important role in lower genital tract anti-HIV immune responses. Method of study: HIV-infected (n = 18) and HIV-uninfected (n = 19) pre-menopausal women participating in the WIHS cohort were recruited. Frequency and phenotype of GD T cells were determined in endocervical cytobrush samples and peripheral blood by multicolor flow cytometry. Results: We found depletion of GD2 cells in the blood of HIV-infected women as well as significant decrease in the frequency of endocervical GD1 cells compared to uninfected women. Conclusion: We report for the first time, the GD1 cells are a predominant endocervical T-cell subset that is significantly decreased in HIV-infected women.

Original language	English (US)
Pages (from-to)	134-145
Number of pages	12
Journal	American Journal of Reproductive Immunology
Volume	75
Issue number	2
DOIs	https://doi.org/10.1111/aji.12458
State	Published - Feb 1 2016

Keywords

Biomarker
Female reproductive tract
Gamma delta T cells
HIV

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Reproductive Medicine
Obstetrics and Gynecology

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10.1111/aji.12458

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title = "Loss of Intra-Epithelial Endocervical Gamma Delta (GD) 1 T Cells in HIV-Infected Women",

abstract = "Problem: Human gamma delta (GD) T cells play a well-documented role in epithelial barrier surveillance and protection. Two subsets of GD T cells, defined by the use of either the Vdelta2 (GD2) or Vdelta1 (GD1) TCR, predominate. We hypothesized that endocervical GD T cells play important role in lower genital tract anti-HIV immune responses. Method of study: HIV-infected (n = 18) and HIV-uninfected (n = 19) pre-menopausal women participating in the WIHS cohort were recruited. Frequency and phenotype of GD T cells were determined in endocervical cytobrush samples and peripheral blood by multicolor flow cytometry. Results: We found depletion of GD2 cells in the blood of HIV-infected women as well as significant decrease in the frequency of endocervical GD1 cells compared to uninfected women. Conclusion: We report for the first time, the GD1 cells are a predominant endocervical T-cell subset that is significantly decreased in HIV-infected women.",

keywords = "Biomarker, Female reproductive tract, Gamma delta T cells, HIV",

author = "Natasa Strbo and Alcaide, {Maria L.} and Laura Romero and Hector Bolivar and Deborah Jones and Podack, {Eckhard R.} and Fischl, {Margaret A.}",

note = "Funding Information: We thank the Miami WIHS study participants for their willingness to participate in this work. Data in this manuscript were collected by the Miami Women''s Interagency HIV Study (WIHS). The contents of this publication are solely the responsibility of the authors and do not represent the official views of the National Institutes of Health (NIH). This work was supported by National Institute of Allergy and Infectious Diseases and Women''s Interagency HIV Infection Study (WIHS) [grant number U01 AI103397], National Institute of Allergy and Infectious Diseases, National Institutes of Health [ grant number P30AI073961], National Center for Advancing Translational Science s and the National Institute on Minority Health and Health Disparities, National Institute of Health [grant number UL1TR000460 and 1KL2TR000461], and National Institute of Child Health and Human Development, National Institute of Health [ grant number K23HD074489]. Publisher Copyright: {\textcopyright} 2016 John Wiley & Sons A/S.",

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doi = "10.1111/aji.12458",

language = "English (US)",

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T1 - Loss of Intra-Epithelial Endocervical Gamma Delta (GD) 1 T Cells in HIV-Infected Women

AU - Strbo, Natasa

AU - Alcaide, Maria L.

AU - Romero, Laura

AU - Bolivar, Hector

AU - Jones, Deborah

AU - Podack, Eckhard R.

AU - Fischl, Margaret A.

N1 - Funding Information: We thank the Miami WIHS study participants for their willingness to participate in this work. Data in this manuscript were collected by the Miami Women''s Interagency HIV Study (WIHS). The contents of this publication are solely the responsibility of the authors and do not represent the official views of the National Institutes of Health (NIH). This work was supported by National Institute of Allergy and Infectious Diseases and Women''s Interagency HIV Infection Study (WIHS) [grant number U01 AI103397], National Institute of Allergy and Infectious Diseases, National Institutes of Health [ grant number P30AI073961], National Center for Advancing Translational Science s and the National Institute on Minority Health and Health Disparities, National Institute of Health [grant number UL1TR000460 and 1KL2TR000461], and National Institute of Child Health and Human Development, National Institute of Health [ grant number K23HD074489]. Publisher Copyright: © 2016 John Wiley & Sons A/S.

PY - 2016/2/1

Y1 - 2016/2/1

N2 - Problem: Human gamma delta (GD) T cells play a well-documented role in epithelial barrier surveillance and protection. Two subsets of GD T cells, defined by the use of either the Vdelta2 (GD2) or Vdelta1 (GD1) TCR, predominate. We hypothesized that endocervical GD T cells play important role in lower genital tract anti-HIV immune responses. Method of study: HIV-infected (n = 18) and HIV-uninfected (n = 19) pre-menopausal women participating in the WIHS cohort were recruited. Frequency and phenotype of GD T cells were determined in endocervical cytobrush samples and peripheral blood by multicolor flow cytometry. Results: We found depletion of GD2 cells in the blood of HIV-infected women as well as significant decrease in the frequency of endocervical GD1 cells compared to uninfected women. Conclusion: We report for the first time, the GD1 cells are a predominant endocervical T-cell subset that is significantly decreased in HIV-infected women.

AB - Problem: Human gamma delta (GD) T cells play a well-documented role in epithelial barrier surveillance and protection. Two subsets of GD T cells, defined by the use of either the Vdelta2 (GD2) or Vdelta1 (GD1) TCR, predominate. We hypothesized that endocervical GD T cells play important role in lower genital tract anti-HIV immune responses. Method of study: HIV-infected (n = 18) and HIV-uninfected (n = 19) pre-menopausal women participating in the WIHS cohort were recruited. Frequency and phenotype of GD T cells were determined in endocervical cytobrush samples and peripheral blood by multicolor flow cytometry. Results: We found depletion of GD2 cells in the blood of HIV-infected women as well as significant decrease in the frequency of endocervical GD1 cells compared to uninfected women. Conclusion: We report for the first time, the GD1 cells are a predominant endocervical T-cell subset that is significantly decreased in HIV-infected women.

KW - Biomarker

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KW - HIV

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SN - 1046-7408

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ER -

Loss of Intra-Epithelial Endocervical Gamma Delta (GD) 1 T Cells in HIV-Infected Women

Abstract

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