Longterm culture of telomerase-transduced rheumatoid arthritis fibroblast-like synoviocytes display a distinct gene expression pattern

Yubo Sun, Gary Firestein, David Boyle, Helen Gruber, Herman S Cheung

Research output: Contribution to journalArticle

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Abstract

Objective. To extend the lifespan of rheumatoid arthritis fibroblast-like synoviocytes (RA FLS) using human telomerase catalytic subunit (hTERT) and to test the hypothesis that longterm culture of hTERT-RA FLS may display a disease-specific gene expression pattern. Methods. RA 516 FLS were transduced by hTERT and the replicative properties of hTERT-RA 516 FLS were evaluated by repeated expansion. Gene expressions in hTERT-RA 516 FLS (passage 8) were compared with the gene expressions in hTERT-osteoarthritis (OA) 13A FLS (passage 8) by microarrays and RT-PCR. After continuous expansion in culture for an additional 4 months, gene expression was examined again using real-time RT-PCR and microarray. Results. While primary RA 516 FLS stopped dividing after repeated culture for about 120 days, hTERT-RA 516 FLS continued to grow at a steady rate. The hTERT-RA 156 FLS displayed a distinct gene expression pattern different from hTERT-OA 13A FLS. Several putative autoantigens and cytokine A2/monocyte chemoattractant protein-1 were expressed at significantly higher levels in longterm culture of hTERT-RA 516 FLS. Conclusion. Telomerase- transduced RA FLS offer an alternative cell model for the study of RA and for examination of cellular/genetic alterations in RA FLS. Our findings provide further support for the notion that RA FLS are altered cells.

Original languageEnglish
Pages (from-to)1959-1970
Number of pages12
JournalJournal of Rheumatology
Volume34
Issue number10
StatePublished - Oct 1 2007

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Telomerase
Rheumatoid Arthritis
Fibroblasts
Gene Expression
Osteoarthritis
Synoviocytes
Chemokine CCL2
Autoantigens
varespladib methyl
Real-Time Polymerase Chain Reaction
Cytokines
Polymerase Chain Reaction

Keywords

  • AGC1
  • Autoantigen
  • CCL2
  • CHI3L1
  • HAPLN1
  • PRG4
  • Rheumatoid arthritis
  • Synoviocytes
  • Telomerase

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

Longterm culture of telomerase-transduced rheumatoid arthritis fibroblast-like synoviocytes display a distinct gene expression pattern. / Sun, Yubo; Firestein, Gary; Boyle, David; Gruber, Helen; Cheung, Herman S.

In: Journal of Rheumatology, Vol. 34, No. 10, 01.10.2007, p. 1959-1970.

Research output: Contribution to journalArticle

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