Long-term survival of rat cardiac allografts by intrathymic plus portal venous injections of donor bone marrow cells and short-term tacrolimus immunosuppression

Johji Fukada, Yoshihiko Kurimoto, Phillip Ruiz, Abdelouahab Aitouche, Adriana Zeevi, S. Li, Toru Kubota, S. M. Pham

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Intrathymic (IT) or portal venous (PV) injection of donor antigens has been shown to prolong organ acceptance in low responder rat strain combinations. We determined whether a combination of these strategies would prolong cardiac allograft survival in high responder combinations. Wistar Furth rats received 1 × 108 ACI rat bone marrow cells (BMCS) via IT, intravenous (IV), PV, IV + PV, IT + IV or IT + PV route at the time of ACI cardiac transplantation. Without tacrolimus (FK), all grafts were acutely rejected. With FK immunosuppression (1.5 mg/kg per day, I.M., days 04), single BMC injection did not increase graft survival beyond 93 days, whereas 70% of grafts survived indefinitely (> 150 days) when IT and PV BMCs were combined. Animals receiving IT and PV BMCs also had less allograft vasculopathy. Thus, IT and PV injections of donor BMCs under a brief course of FK synergistically improve cardiac allograft survival.

Original languageEnglish
Pages (from-to)311-319
Number of pages9
JournalTransplant International
Volume14
Issue number5
DOIs
StatePublished - Nov 12 2001

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Keywords

  • Allografts vasculopathy
  • Bone marrow cells
  • Intrathymic injection
  • Portal venous injection
  • Rat cardiac allograft

ASJC Scopus subject areas

  • Transplantation

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