Long-term survival of glial segments during nerve regeneration in the leech

Ellen J. Elliot, Kenneth J Muller

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Nerve injury that severs axons also disrupts ensheathing glial cells. Specifically, crushing or cutting the leech nerve cord separates the glial cell's nucleated portion from an anucleate segment, or `stump'. In experiments reported here, the fate of the stump and the response of the nucleated portion were determined by intracellular recording, by intracellular injection of Lucifer Yellow dye and horseradish peroxidase (HRP) as tracers, and by electron microscopy. The nucleated portion of the glial cell did not divide, degenerate, or grow appreciably. The severed glial stump remained isolated from the nucleated portion but maintained its resting potential and normal morphology for months. Stumps typically began to deteriorate after 3 months. Small macrophage-like cells, or 'microglia' increased in number after injury and ensheathed axons, thus partially replacing the atrophying glial stump. Some axons in the nerve cord degenerated; the remainder appeared morphologically and physiologically normal. Thus, both nucleated and anucleate glial segments persisted throughout the one to two months required for axons to regenerate functional connections. Glial cells in the leech are therefore available to guide physically the growing axons or to contribute in other ways to nerve regeneration.

Original languageEnglish
Pages (from-to)99-113
Number of pages15
JournalBrain Research
Volume218
Issue number1-2
DOIs
StatePublished - Aug 10 1981
Externally publishedYes

Fingerprint

Leeches
Nerve Regeneration
Neuroglia
Axons
Wounds and Injuries
Microglia
Horseradish Peroxidase
Membrane Potentials
Electron Microscopy
Coloring Agents
Macrophages
Injections

Keywords

  • glial segment survival
  • leech nerve cord
  • nerve regeneration

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Long-term survival of glial segments during nerve regeneration in the leech. / Elliot, Ellen J.; Muller, Kenneth J.

In: Brain Research, Vol. 218, No. 1-2, 10.08.1981, p. 99-113.

Research output: Contribution to journalArticle

@article{ab8c7f865eea4827b34160c3178858c3,
title = "Long-term survival of glial segments during nerve regeneration in the leech",
abstract = "Nerve injury that severs axons also disrupts ensheathing glial cells. Specifically, crushing or cutting the leech nerve cord separates the glial cell's nucleated portion from an anucleate segment, or `stump'. In experiments reported here, the fate of the stump and the response of the nucleated portion were determined by intracellular recording, by intracellular injection of Lucifer Yellow dye and horseradish peroxidase (HRP) as tracers, and by electron microscopy. The nucleated portion of the glial cell did not divide, degenerate, or grow appreciably. The severed glial stump remained isolated from the nucleated portion but maintained its resting potential and normal morphology for months. Stumps typically began to deteriorate after 3 months. Small macrophage-like cells, or 'microglia' increased in number after injury and ensheathed axons, thus partially replacing the atrophying glial stump. Some axons in the nerve cord degenerated; the remainder appeared morphologically and physiologically normal. Thus, both nucleated and anucleate glial segments persisted throughout the one to two months required for axons to regenerate functional connections. Glial cells in the leech are therefore available to guide physically the growing axons or to contribute in other ways to nerve regeneration.",
keywords = "glial segment survival, leech nerve cord, nerve regeneration",
author = "Elliot, {Ellen J.} and Muller, {Kenneth J}",
year = "1981",
month = "8",
day = "10",
doi = "10.1016/0006-8993(81)90991-4",
language = "English",
volume = "218",
pages = "99--113",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Long-term survival of glial segments during nerve regeneration in the leech

AU - Elliot, Ellen J.

AU - Muller, Kenneth J

PY - 1981/8/10

Y1 - 1981/8/10

N2 - Nerve injury that severs axons also disrupts ensheathing glial cells. Specifically, crushing or cutting the leech nerve cord separates the glial cell's nucleated portion from an anucleate segment, or `stump'. In experiments reported here, the fate of the stump and the response of the nucleated portion were determined by intracellular recording, by intracellular injection of Lucifer Yellow dye and horseradish peroxidase (HRP) as tracers, and by electron microscopy. The nucleated portion of the glial cell did not divide, degenerate, or grow appreciably. The severed glial stump remained isolated from the nucleated portion but maintained its resting potential and normal morphology for months. Stumps typically began to deteriorate after 3 months. Small macrophage-like cells, or 'microglia' increased in number after injury and ensheathed axons, thus partially replacing the atrophying glial stump. Some axons in the nerve cord degenerated; the remainder appeared morphologically and physiologically normal. Thus, both nucleated and anucleate glial segments persisted throughout the one to two months required for axons to regenerate functional connections. Glial cells in the leech are therefore available to guide physically the growing axons or to contribute in other ways to nerve regeneration.

AB - Nerve injury that severs axons also disrupts ensheathing glial cells. Specifically, crushing or cutting the leech nerve cord separates the glial cell's nucleated portion from an anucleate segment, or `stump'. In experiments reported here, the fate of the stump and the response of the nucleated portion were determined by intracellular recording, by intracellular injection of Lucifer Yellow dye and horseradish peroxidase (HRP) as tracers, and by electron microscopy. The nucleated portion of the glial cell did not divide, degenerate, or grow appreciably. The severed glial stump remained isolated from the nucleated portion but maintained its resting potential and normal morphology for months. Stumps typically began to deteriorate after 3 months. Small macrophage-like cells, or 'microglia' increased in number after injury and ensheathed axons, thus partially replacing the atrophying glial stump. Some axons in the nerve cord degenerated; the remainder appeared morphologically and physiologically normal. Thus, both nucleated and anucleate glial segments persisted throughout the one to two months required for axons to regenerate functional connections. Glial cells in the leech are therefore available to guide physically the growing axons or to contribute in other ways to nerve regeneration.

KW - glial segment survival

KW - leech nerve cord

KW - nerve regeneration

UR - http://www.scopus.com/inward/record.url?scp=0019411033&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019411033&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(81)90991-4

DO - 10.1016/0006-8993(81)90991-4

M3 - Article

VL - 218

SP - 99

EP - 113

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -