Long-term expression of a fluorescent reporter gene via direct injection of plasmid vector into mouse skeletal muscle

Comparison of human creatine kinase and CMV promoter expression levels in vivo

R. J. Bartlett, S. L. Secore, J. T. Singer, M. Bodo, Khema R Sharma, Camillo Ricordi

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Expression of a fluorescent reporter gene has been studied using two alternate promoters to transcribe the green fluorescent protein (gfp) from Aequorea victoria. The human cytomegalovirus (CMV) enhancer/promoter or the human muscle-specific creatine kinase promoter (CKM) were inserted along with the gfp cDNA into a plasmid expression vector based on a modified adeno- associated virus genome. Naked plasmid DNA was injected into the hamstring muscle of mdx mice and gfp gene expression determined from frozen muscle sections taken at 4, 14, and 42 days postinjection. Fluorescence patterns obtained by photomicroscopy and quantitative fluorescence measurements indicated a near-linear increase in the accumulation of the gfp in skeletal muscle during the length of the study, with gfp expression at 42 days being roughly four times the values obtained at 4 days. The levels of expression of gfp from the CKM construct were consistently higher than for the CMV construct. The CKM promoter/expression vector combination demonstrates significant potential for simple, direct delivery and long-term, high-level expression of genes in skeletal muscle.

Original languageEnglish
Pages (from-to)411-419
Number of pages9
JournalCell Transplantation
Volume5
Issue number3
DOIs
StatePublished - May 1 1996

Fingerprint

Direct injection
Creatine Kinase
Green Fluorescent Proteins
Cytomegalovirus
Reporter Genes
Muscle
Skeletal Muscle
Plasmids
Genes
Proteins
Injections
Fluorescence
MM Form Creatine Kinase
Inbred mdx Mouse
Gene Expression
Dependovirus
Frozen Sections
Protein Kinases
Viruses
Gene expression

Keywords

  • Adeno- associated virus
  • CMV promoter/enhancer
  • Green fluorescent protein
  • Human creatine kinase promoter
  • Reporter gene expression
  • Skeletal muscle

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

Cite this

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abstract = "Expression of a fluorescent reporter gene has been studied using two alternate promoters to transcribe the green fluorescent protein (gfp) from Aequorea victoria. The human cytomegalovirus (CMV) enhancer/promoter or the human muscle-specific creatine kinase promoter (CKM) were inserted along with the gfp cDNA into a plasmid expression vector based on a modified adeno- associated virus genome. Naked plasmid DNA was injected into the hamstring muscle of mdx mice and gfp gene expression determined from frozen muscle sections taken at 4, 14, and 42 days postinjection. Fluorescence patterns obtained by photomicroscopy and quantitative fluorescence measurements indicated a near-linear increase in the accumulation of the gfp in skeletal muscle during the length of the study, with gfp expression at 42 days being roughly four times the values obtained at 4 days. The levels of expression of gfp from the CKM construct were consistently higher than for the CMV construct. The CKM promoter/expression vector combination demonstrates significant potential for simple, direct delivery and long-term, high-level expression of genes in skeletal muscle.",
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