Long-term bone marrow culture and its clinical potential in chronic wound healing

Evangelos V Badiavas, Dwayne Ford, Paul Liu, Nicola Kouttab, John Morgan, Amy Richards, Abby Maizel

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Bone marrow-derived cells have long been regarded to play a crucial role in the homeostasis of skin. We have previously described the clinical benefit of directly applying autologous bone marrow aspirate and cultured bone marrow cells to recalcitrant chronic skin wounds. The initial response to treatment appears to be vascular in nature with the formation of new blood vessels. The difficulty in consistently growing adequate numbers of cells for delivery to patients was, however, a limiting factor. Here, in a subsequent protocol, we describe an improved bone marrow culture system yielding a reliable growth of bone marrow cells and leading to a greater clinical response. Cells expressing markers of endothelial progenitors including CD133, CD146, and particularly CD14 are enhanced in these cultures. CD14-isolated cells produced colonies in endothelial cell assays and sprouting in matrigel assays. Angiogenic cytokines, including angiogenin, epithelial neutrophil-activating protein-78, growth-regulated oncogene, growth-regulated oncogene-α, Interleukin-8, CXC16, and monocyte chemoattractant protein-1, were found to be elevated in these cultures. Administration of improved culture cells to patients with chronic wounds present for >1 year lead to an enhanced clinical response.

Original languageEnglish
Pages (from-to)856-865
Number of pages10
JournalWound Repair and Regeneration
Volume15
Issue number6
DOIs
StatePublished - Nov 1 2007
Externally publishedYes

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Bone Marrow Cells
Wound Healing
Bone Marrow
Oncogenes
Blood Vessels
Growth
Skin
Chemokine CCL2
Wounds and Injuries
Interleukin-8
Cultured Cells
Neutrophils
Homeostasis
Endothelial Cells
Cell Culture Techniques
Cell Count
Cytokines
Proteins
Therapeutics
angiogenin

ASJC Scopus subject areas

  • Dermatology
  • Surgery

Cite this

Long-term bone marrow culture and its clinical potential in chronic wound healing. / Badiavas, Evangelos V; Ford, Dwayne; Liu, Paul; Kouttab, Nicola; Morgan, John; Richards, Amy; Maizel, Abby.

In: Wound Repair and Regeneration, Vol. 15, No. 6, 01.11.2007, p. 856-865.

Research output: Contribution to journalArticle

Badiavas, Evangelos V ; Ford, Dwayne ; Liu, Paul ; Kouttab, Nicola ; Morgan, John ; Richards, Amy ; Maizel, Abby. / Long-term bone marrow culture and its clinical potential in chronic wound healing. In: Wound Repair and Regeneration. 2007 ; Vol. 15, No. 6. pp. 856-865.
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