Localized maternal mRNA related to transforming growth factor β mRNA is concentrated in a cytokeratin-enriched fraction from Xenopus oocytes

M. D. Pondel, M. L. King

Research output: Contribution to journalArticlepeer-review

88 Scopus citations

Abstract

The localized maternal RNA Vg1 resides in the cortical region of the vegetal pole of fully grown Xenopus oocytes and is inherited by only a subset of blastomeres in the early embryo [Weeks, D.L. & Melton, D.A. (1987) Cell 51, 861-867]. Because RNA-cyttoskeletal interactions may play a role in RNA localization, we have examined the association of Vg1 RNA with components of the oocyte's cytoskeleton. Gel and immunoblot analysis of a detergent-insoluble fraction revealed a greatly simplified protein pattern composed largely of cytokeratins and vimentin. In sharp contrast to the nonlocalized histone H3 mRNA, Vg1 RNA was concentrated some 35- to 50-fold in this insoluble fraction. Extractions at higher salt concentrations yielded preparations further enriched in cytokeratins and in the Vg1 RNA. Upon ovulation, Vg1 RNA is released into the soluble fraction. This change in Vg1 RNA distribution coincides with the observed breakdown of cortical cytokeratin filaments [Klymkowsky, M.W., Maynell, L.A. & Polson, A.G. (1987) Development 100, 543-557] and the loss of Vg1 RNA from the cortical region. Our findings are consistent with the hypothesis that RNA-cytoskeletal interactions are involved in the localization and segregation of information during development.

Original languageEnglish (US)
Pages (from-to)7612-7616
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number20
DOIs
StatePublished - 1988

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Localized maternal mRNA related to transforming growth factor β mRNA is concentrated in a cytokeratin-enriched fraction from Xenopus oocytes'. Together they form a unique fingerprint.

Cite this