Localization of the pannexin1 protein at postsynaptic sites in the cerebral cortex and hippocampus

G. Zoidl, E. Petrasch-Parwez, A. Ray, C. Meier, S. Bunse, H. W. Habbes, G. Dahl, R. Dermietzel

Research output: Contribution to journalArticle

106 Scopus citations

Abstract

Pannexins (Panx) constitute a new family of gap junction type proteins. Functional expression in paired Xenopus oocytes indicated that pannexins are capable of forming communicating junctions but also proved to be active in forming of unapposed hemichannels. In the vertebrate brain pannexins have been found in neurons. However, the subcellular cerebral localization of pannexin proteins which could gain first clues on their putative function is essentially unknown. Here we demonstrate by light and electron microscopical immunohistochemistry that Panx1 reveals postsynaptic localization in rodent hippocampal and cortical principal neurons accumulating at postsynaptic densities. The postsynaptic localization was corroborated by co-localization of Panx1 with postsynaptic density protein 95 (PSD-95), a prominent postsynaptic scaffolding protein, in hippocampal neurons expressing tagged versions of these proteins. The asymmetric synaptic distribution of Panx1 suggests that it may function in neurons as non-junctional channels (pannexons) at postsynaptic sites and comprises a novel component of the postsynaptic protein complex.

Original languageEnglish (US)
Pages (from-to)9-16
Number of pages8
JournalNeuroscience
Volume146
Issue number1
DOIs
StatePublished - Apr 25 2007

Keywords

  • brain tissue
  • gap junction
  • hemichannel
  • pannexin
  • PSD-95

ASJC Scopus subject areas

  • Neuroscience(all)

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    Zoidl, G., Petrasch-Parwez, E., Ray, A., Meier, C., Bunse, S., Habbes, H. W., Dahl, G., & Dermietzel, R. (2007). Localization of the pannexin1 protein at postsynaptic sites in the cerebral cortex and hippocampus. Neuroscience, 146(1), 9-16. https://doi.org/10.1016/j.neuroscience.2007.01.061