Abstract
Translocation (15;17)(q22;q12-q21) is a chromosome aberration specifically found in acute promyelocytic leukemia (APL), that generates a chimeric gene between the promyelocytic leukemia (PML) gene on chromosome 15 and the retinoic acid receptor α (RARA) gene, on chromosome 17. In the course of molecular investigations of a series of 28 Chinese patients with APL, we have simultaneously used Southern blot and reverse transcriptase polymerase chain reaction (RT-PCR) analysis to characterize the PML gene breakpoints on chromosome 15 and identify PML-RARA fusion transcripts. Our results confirmed the existence of the three recently described bcrl, bcr2, and bcr3 breakpoint cluster regions. In addition, structural data provided by PML-RARA transcripts allowed us to more accurately locate the 3′ borders of clusters bcM and bcr3. Moreover, our data suggest a preferential localization of the breakpoints within bcrl and bcr3. The primary structure of a 1.4 kb DNA segment flanking the 5′ part of the PML gene and that of the ber3 cluster (2.1 kb) were also established.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 20-26 |
| Number of pages | 7 |
| Journal | Leukemia |
| Volume | 7 |
| Issue number | 1 |
| State | Published - Jan 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cancer Research
- Hematology
Cite this
Localization of the chromosome 15 breakpoints and expression of multiple PML-RARα transcripts in acute promyelocytic leukemia : A study of 28 Chinese patients. / Geng, Jie Ping; Tong, Jian Hua; Dong, Shuo; Wang, Zhen Yi; Chen, Sai Juan; Chen, Zhu; Zelent, Arthur Z; Berger, Roland; Larsen, Christian Jacques.
In: Leukemia, Vol. 7, No. 1, 01.1993, p. 20-26.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Localization of the chromosome 15 breakpoints and expression of multiple PML-RARα transcripts in acute promyelocytic leukemia
T2 - A study of 28 Chinese patients
AU - Geng, Jie Ping
AU - Tong, Jian Hua
AU - Dong, Shuo
AU - Wang, Zhen Yi
AU - Chen, Sai Juan
AU - Chen, Zhu
AU - Zelent, Arthur Z
AU - Berger, Roland
AU - Larsen, Christian Jacques
PY - 1993/1
Y1 - 1993/1
N2 - Translocation (15;17)(q22;q12-q21) is a chromosome aberration specifically found in acute promyelocytic leukemia (APL), that generates a chimeric gene between the promyelocytic leukemia (PML) gene on chromosome 15 and the retinoic acid receptor α (RARA) gene, on chromosome 17. In the course of molecular investigations of a series of 28 Chinese patients with APL, we have simultaneously used Southern blot and reverse transcriptase polymerase chain reaction (RT-PCR) analysis to characterize the PML gene breakpoints on chromosome 15 and identify PML-RARA fusion transcripts. Our results confirmed the existence of the three recently described bcrl, bcr2, and bcr3 breakpoint cluster regions. In addition, structural data provided by PML-RARA transcripts allowed us to more accurately locate the 3′ borders of clusters bcM and bcr3. Moreover, our data suggest a preferential localization of the breakpoints within bcrl and bcr3. The primary structure of a 1.4 kb DNA segment flanking the 5′ part of the PML gene and that of the ber3 cluster (2.1 kb) were also established.
AB - Translocation (15;17)(q22;q12-q21) is a chromosome aberration specifically found in acute promyelocytic leukemia (APL), that generates a chimeric gene between the promyelocytic leukemia (PML) gene on chromosome 15 and the retinoic acid receptor α (RARA) gene, on chromosome 17. In the course of molecular investigations of a series of 28 Chinese patients with APL, we have simultaneously used Southern blot and reverse transcriptase polymerase chain reaction (RT-PCR) analysis to characterize the PML gene breakpoints on chromosome 15 and identify PML-RARA fusion transcripts. Our results confirmed the existence of the three recently described bcrl, bcr2, and bcr3 breakpoint cluster regions. In addition, structural data provided by PML-RARA transcripts allowed us to more accurately locate the 3′ borders of clusters bcM and bcr3. Moreover, our data suggest a preferential localization of the breakpoints within bcrl and bcr3. The primary structure of a 1.4 kb DNA segment flanking the 5′ part of the PML gene and that of the ber3 cluster (2.1 kb) were also established.
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UR - http://www.scopus.com/inward/citedby.url?scp=0027409955&partnerID=8YFLogxK
M3 - Article
C2 - 8380300
AN - SCOPUS:0027409955
VL - 7
SP - 20
EP - 26
JO - Leukemia
JF - Leukemia
SN - 0887-6924
IS - 1
ER -