Localization of the chromosome 15 breakpoints and expression of multiple PML-RARα transcripts in acute promyelocytic leukemia: A study of 28 Chinese patients

Jie Ping Geng, Jian Hua Tong, Shuo Dong, Zhen Yi Wang, Sai Juan Chen, Zhu Chen, Arthur Z Zelent, Roland Berger, Christian Jacques Larsen

Research output: Contribution to journalArticle

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Abstract

Translocation (15;17)(q22;q12-q21) is a chromosome aberration specifically found in acute promyelocytic leukemia (APL), that generates a chimeric gene between the promyelocytic leukemia (PML) gene on chromosome 15 and the retinoic acid receptor α (RARA) gene, on chromosome 17. In the course of molecular investigations of a series of 28 Chinese patients with APL, we have simultaneously used Southern blot and reverse transcriptase polymerase chain reaction (RT-PCR) analysis to characterize the PML gene breakpoints on chromosome 15 and identify PML-RARA fusion transcripts. Our results confirmed the existence of the three recently described bcrl, bcr2, and bcr3 breakpoint cluster regions. In addition, structural data provided by PML-RARA transcripts allowed us to more accurately locate the 3′ borders of clusters bcM and bcr3. Moreover, our data suggest a preferential localization of the breakpoints within bcrl and bcr3. The primary structure of a 1.4 kb DNA segment flanking the 5′ part of the PML gene and that of the ber3 cluster (2.1 kb) were also established.

Original languageEnglish (US)
Pages (from-to)20-26
Number of pages7
JournalLeukemia
Volume7
Issue number1
StatePublished - Jan 1993
Externally publishedYes

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Chromosome Breakpoints
Chromosomes, Human, Pair 15
Acute Promyelocytic Leukemia
Leukemia
Genes
Chromosomes, Human, Pair 17
Retinoic Acid Receptors
Multigene Family
Southern Blotting
Reverse Transcriptase Polymerase Chain Reaction
Chromosome Aberrations
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Hematology

Cite this

Geng, J. P., Tong, J. H., Dong, S., Wang, Z. Y., Chen, S. J., Chen, Z., ... Larsen, C. J. (1993). Localization of the chromosome 15 breakpoints and expression of multiple PML-RARα transcripts in acute promyelocytic leukemia: A study of 28 Chinese patients. Leukemia, 7(1), 20-26.

Localization of the chromosome 15 breakpoints and expression of multiple PML-RARα transcripts in acute promyelocytic leukemia : A study of 28 Chinese patients. / Geng, Jie Ping; Tong, Jian Hua; Dong, Shuo; Wang, Zhen Yi; Chen, Sai Juan; Chen, Zhu; Zelent, Arthur Z; Berger, Roland; Larsen, Christian Jacques.

In: Leukemia, Vol. 7, No. 1, 01.1993, p. 20-26.

Research output: Contribution to journalArticle

Geng, JP, Tong, JH, Dong, S, Wang, ZY, Chen, SJ, Chen, Z, Zelent, AZ, Berger, R & Larsen, CJ 1993, 'Localization of the chromosome 15 breakpoints and expression of multiple PML-RARα transcripts in acute promyelocytic leukemia: A study of 28 Chinese patients', Leukemia, vol. 7, no. 1, pp. 20-26.
Geng, Jie Ping ; Tong, Jian Hua ; Dong, Shuo ; Wang, Zhen Yi ; Chen, Sai Juan ; Chen, Zhu ; Zelent, Arthur Z ; Berger, Roland ; Larsen, Christian Jacques. / Localization of the chromosome 15 breakpoints and expression of multiple PML-RARα transcripts in acute promyelocytic leukemia : A study of 28 Chinese patients. In: Leukemia. 1993 ; Vol. 7, No. 1. pp. 20-26.
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