Localization of the chromosome 15 breakpoints and expression of multiple PML-RARα transcripts in acute promyelocytic leukemia: A study of 28 Chinese patients

Jie Ping Geng, Jian Hua Tong, Shuo Dong, Zhen Yi Wang, Sai Juan Chen, Zhu Chen, Arthur Zelent, Roland Berger, Christian Jacques Larsen

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Translocation (15;17)(q22;q12-q21) is a chromosome aberration specifically found in acute promyelocytic leukemia (APL), that generates a chimeric gene between the promyelocytic leukemia (PML) gene on chromosome 15 and the retinoic acid receptor α (RARA) gene, on chromosome 17. In the course of molecular investigations of a series of 28 Chinese patients with APL, we have simultaneously used Southern blot and reverse transcriptase polymerase chain reaction (RT-PCR) analysis to characterize the PML gene breakpoints on chromosome 15 and identify PML-RARA fusion transcripts. Our results confirmed the existence of the three recently described bcrl, bcr2, and bcr3 breakpoint cluster regions. In addition, structural data provided by PML-RARA transcripts allowed us to more accurately locate the 3′ borders of clusters bcM and bcr3. Moreover, our data suggest a preferential localization of the breakpoints within bcrl and bcr3. The primary structure of a 1.4 kb DNA segment flanking the 5′ part of the PML gene and that of the ber3 cluster (2.1 kb) were also established.

Original languageEnglish (US)
Pages (from-to)20-26
Number of pages7
JournalLeukemia
Volume7
Issue number1
StatePublished - Jan 1993

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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    Geng, J. P., Tong, J. H., Dong, S., Wang, Z. Y., Chen, S. J., Chen, Z., Zelent, A., Berger, R., & Larsen, C. J. (1993). Localization of the chromosome 15 breakpoints and expression of multiple PML-RARα transcripts in acute promyelocytic leukemia: A study of 28 Chinese patients. Leukemia, 7(1), 20-26.