Localization of S protein and its relationship to the membrane attack complex of complement in renal tissue

R. J. Falk, E. Podack, A. P. Dalmasso, J. C. Jennette

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The S protein (S) binds to the attack complex of complement (C5b-9) in plasma preventing cytolysis. Using immunofluorescence microscopy, the authors determined the distribution of S in human renal tissue and its relationship to C5b-9, immunoglobulins, C3, albumin, and fibronectin. They examined normal and diseased human kidney tissue from patients with several forms of glomerulonephritis, diabetic nephropathy, and arterionephrosclerosis. S and C5b-9 were found in all diseased tissues; their amounts and distribution directly correlated with severity and location of injury. S and C5b-9 were colocalized in all immune deposits and in all injured glomeruli, tubular basement membranes, and vessel walls. Other than within immune deposits, S and C5b-9 were usually not colocalized with C3. This study demonstrates that S is deposited in areas of tissue injury and thus may participate in the pathogenesis of renal damage. Because in tissue S and C5b-9 are always associated, the attack complex in tissue must either be derived from the circulation as SC5b-9 or it must be capable of binding S after the formation in situ of C5b-9.

Original languageEnglish
Pages (from-to)182-190
Number of pages9
JournalAmerican Journal of Pathology
Volume127
Issue number1
StatePublished - Jan 1 1987
Externally publishedYes

Fingerprint

Complement Membrane Attack Complex
Protein S
Kidney
Wounds and Injuries
Kidney Diseases
Diabetic Nephropathies
Glomerulonephritis
Fibronectins
Fluorescence Microscopy
Basement Membrane
Immunoglobulins
Albumins

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Localization of S protein and its relationship to the membrane attack complex of complement in renal tissue. / Falk, R. J.; Podack, E.; Dalmasso, A. P.; Jennette, J. C.

In: American Journal of Pathology, Vol. 127, No. 1, 01.01.1987, p. 182-190.

Research output: Contribution to journalArticle

Falk, R. J. ; Podack, E. ; Dalmasso, A. P. ; Jennette, J. C. / Localization of S protein and its relationship to the membrane attack complex of complement in renal tissue. In: American Journal of Pathology. 1987 ; Vol. 127, No. 1. pp. 182-190.
@article{225a223dc5b147b3936096cb26f5f37c,
title = "Localization of S protein and its relationship to the membrane attack complex of complement in renal tissue",
abstract = "The S protein (S) binds to the attack complex of complement (C5b-9) in plasma preventing cytolysis. Using immunofluorescence microscopy, the authors determined the distribution of S in human renal tissue and its relationship to C5b-9, immunoglobulins, C3, albumin, and fibronectin. They examined normal and diseased human kidney tissue from patients with several forms of glomerulonephritis, diabetic nephropathy, and arterionephrosclerosis. S and C5b-9 were found in all diseased tissues; their amounts and distribution directly correlated with severity and location of injury. S and C5b-9 were colocalized in all immune deposits and in all injured glomeruli, tubular basement membranes, and vessel walls. Other than within immune deposits, S and C5b-9 were usually not colocalized with C3. This study demonstrates that S is deposited in areas of tissue injury and thus may participate in the pathogenesis of renal damage. Because in tissue S and C5b-9 are always associated, the attack complex in tissue must either be derived from the circulation as SC5b-9 or it must be capable of binding S after the formation in situ of C5b-9.",
author = "Falk, {R. J.} and E. Podack and Dalmasso, {A. P.} and Jennette, {J. C.}",
year = "1987",
month = "1",
day = "1",
language = "English",
volume = "127",
pages = "182--190",
journal = "American Journal of Pathology",
issn = "0002-9440",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Localization of S protein and its relationship to the membrane attack complex of complement in renal tissue

AU - Falk, R. J.

AU - Podack, E.

AU - Dalmasso, A. P.

AU - Jennette, J. C.

PY - 1987/1/1

Y1 - 1987/1/1

N2 - The S protein (S) binds to the attack complex of complement (C5b-9) in plasma preventing cytolysis. Using immunofluorescence microscopy, the authors determined the distribution of S in human renal tissue and its relationship to C5b-9, immunoglobulins, C3, albumin, and fibronectin. They examined normal and diseased human kidney tissue from patients with several forms of glomerulonephritis, diabetic nephropathy, and arterionephrosclerosis. S and C5b-9 were found in all diseased tissues; their amounts and distribution directly correlated with severity and location of injury. S and C5b-9 were colocalized in all immune deposits and in all injured glomeruli, tubular basement membranes, and vessel walls. Other than within immune deposits, S and C5b-9 were usually not colocalized with C3. This study demonstrates that S is deposited in areas of tissue injury and thus may participate in the pathogenesis of renal damage. Because in tissue S and C5b-9 are always associated, the attack complex in tissue must either be derived from the circulation as SC5b-9 or it must be capable of binding S after the formation in situ of C5b-9.

AB - The S protein (S) binds to the attack complex of complement (C5b-9) in plasma preventing cytolysis. Using immunofluorescence microscopy, the authors determined the distribution of S in human renal tissue and its relationship to C5b-9, immunoglobulins, C3, albumin, and fibronectin. They examined normal and diseased human kidney tissue from patients with several forms of glomerulonephritis, diabetic nephropathy, and arterionephrosclerosis. S and C5b-9 were found in all diseased tissues; their amounts and distribution directly correlated with severity and location of injury. S and C5b-9 were colocalized in all immune deposits and in all injured glomeruli, tubular basement membranes, and vessel walls. Other than within immune deposits, S and C5b-9 were usually not colocalized with C3. This study demonstrates that S is deposited in areas of tissue injury and thus may participate in the pathogenesis of renal damage. Because in tissue S and C5b-9 are always associated, the attack complex in tissue must either be derived from the circulation as SC5b-9 or it must be capable of binding S after the formation in situ of C5b-9.

UR - http://www.scopus.com/inward/record.url?scp=0023223050&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023223050&partnerID=8YFLogxK

M3 - Article

C2 - 2952015

AN - SCOPUS:0023223050

VL - 127

SP - 182

EP - 190

JO - American Journal of Pathology

JF - American Journal of Pathology

SN - 0002-9440

IS - 1

ER -