Localization of polyketide synthase encoding genes to the toxic dinoflagellate Karenia brevis

Richard V. Snyder, Maria A. Guerrero, Christopher D. Sinigalliano, Jamie Winshell, Roberto Perez, Jose V. Lopez, Kathleen S. Rein

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Karenia brevis is a toxic marine dinoflagellate endemic to the Gulf of Mexico. Blooms of this harmful alga cause fish kills, marine mammal mortalities and neurotoxic shellfish poisonings. These harmful effects are attributed to a suite of polyketide secondary metabolites known as the brevetoxins. The carbon framework of all polyketides is assembled by a polyketide synthase (PKS). Previously, PKS encoding genes were amplified from K. brevis culture and their similarity to a PKS gene from the closely related protist, Cryptosporidium parvum, suggested that these genes originate from the dinoflagellate. However, K. brevis has not been grown axenically. The associated bacteria might be the source of the toxins or the PKS genes. Herein we report the localization of PKS encoding genes by a combination of flow cytometry/PCR and fluorescence in situ hybridization (FISH). Two genes localized exclusively to K. brevis cells while a third localized to both K. brevis and associated bacteria. While these genes have not yet been linked to toxin production, the work describes the first definitive evidence of resident PKS genes in any dinoflagellate.

Original languageEnglish
Pages (from-to)1767-1780
Number of pages14
JournalPhytochemistry
Volume66
Issue number15
DOIs
StatePublished - Aug 1 2005

Fingerprint

Polyketide Synthases
polyketide synthases
Dinoflagellida
Gene encoding
Poisons
Genes
Polyketides
genes
polyketides
Bacteria
Shellfish
neurotoxic shellfish poisoning
Mammals
toxins
Flow cytometry
Shellfish Poisoning
brevetoxins
Gulf of Mexico
Algae
Metabolites

Keywords

  • Brevetoxin
  • Dinoflagellate
  • Fluorescence in situ hybridization
  • Karenia brevis
  • Polyketide synthase

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery

Cite this

Snyder, R. V., Guerrero, M. A., Sinigalliano, C. D., Winshell, J., Perez, R., Lopez, J. V., & Rein, K. S. (2005). Localization of polyketide synthase encoding genes to the toxic dinoflagellate Karenia brevis. Phytochemistry, 66(15), 1767-1780. https://doi.org/10.1016/j.phytochem.2005.06.010

Localization of polyketide synthase encoding genes to the toxic dinoflagellate Karenia brevis. / Snyder, Richard V.; Guerrero, Maria A.; Sinigalliano, Christopher D.; Winshell, Jamie; Perez, Roberto; Lopez, Jose V.; Rein, Kathleen S.

In: Phytochemistry, Vol. 66, No. 15, 01.08.2005, p. 1767-1780.

Research output: Contribution to journalArticle

Snyder, RV, Guerrero, MA, Sinigalliano, CD, Winshell, J, Perez, R, Lopez, JV & Rein, KS 2005, 'Localization of polyketide synthase encoding genes to the toxic dinoflagellate Karenia brevis', Phytochemistry, vol. 66, no. 15, pp. 1767-1780. https://doi.org/10.1016/j.phytochem.2005.06.010
Snyder RV, Guerrero MA, Sinigalliano CD, Winshell J, Perez R, Lopez JV et al. Localization of polyketide synthase encoding genes to the toxic dinoflagellate Karenia brevis. Phytochemistry. 2005 Aug 1;66(15):1767-1780. https://doi.org/10.1016/j.phytochem.2005.06.010
Snyder, Richard V. ; Guerrero, Maria A. ; Sinigalliano, Christopher D. ; Winshell, Jamie ; Perez, Roberto ; Lopez, Jose V. ; Rein, Kathleen S. / Localization of polyketide synthase encoding genes to the toxic dinoflagellate Karenia brevis. In: Phytochemistry. 2005 ; Vol. 66, No. 15. pp. 1767-1780.
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