Localization of enteroviral RNA within the pancreas in donors with T1D and T1D-associated autoantibodies

Shirin Geravandi, Sarah Richardson, Alberto Pugliese, Kathrin Maedler

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Enteroviral infections have been associated with autoimmunity and type 1 diabetes (T1D), but reliable methods to ascertain localization of single infected cells in the pancreas were missing. Using a single-molecule-based fluorescent in situ hybridization (smFISH) method, we detected increased virus infection in pancreases from organ donors with T1D and with disease-associated autoantibodies (AAb+). Although virus-positive β cells are found at higher frequency in T1D pancreases, compared to control donors, but are scarce, most virus-positive cells are scattered in the exocrine pancreas. Augmented CD45+ lymphocytes in T1D pancreases show virus positivity or localization in close proximity to virus-positive cells. Many more infected cells were also found in spleens from T1D donors. The overall increased proportion of virus-positive cells in the pancreas of AAb+ and T1D organ donors suggests that enteroviruses are associated with immune cell infiltration, autoimmunity, and β cell destruction in both preclinical and diagnosed T1D.

Original languageEnglish (US)
Article number100371
JournalCell Reports Medicine
Volume2
Issue number8
DOIs
StatePublished - Aug 17 2021

Keywords

  • AAb
  • autoantibodies
  • autoimmunity
  • coxsackievirus
  • enterovirus
  • pancreas
  • smFISH
  • T1D
  • type 1 diabetes
  • β-cell

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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