Localization of cloned unique DNA to three different regions of chromosome 19: Screen for linkage probes for myotonic dystrophy

L. H. Yamaoka, R. J. Bartlett, D. A. Ross, G. H. Fey, D. H. Ledbetter, G. Bruns, M. A. Pericak-Vance, M. H. Herbstreith, A. D. Roses

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Screening polymorphic DNA probes for linkage to myotonic dystrophy (DM) and to other reported chromosome 19 (CH19) genes will develop a linkage map for human CH19. We report here the assignment of 3 cloned unique DNA sequences to 3 distinct regions of CH19. The novel use of 35S-labeled probes facilitated the rapid localization of the gene for the third complement factor (C3) to 19pl3.2 by in situ hybridization. Metaphase chromosomes were from normal peripheral lymphocytes as well as from a fibroblast line containing a 15;19 translocation which permitted clear identification of CH19 regions of localization. Two random clones isolated from a plasmid library of human F-group enriched chromosomal DNA (D19S5 and D19S6) were in like manner assigned to 19pl.2 and 19ql3.2 to 19qter, respectively.

Original languageEnglish (US)
Pages (from-to)403-412
Number of pages10
JournalJournal of Neurogenetics
Volume2
Issue number6
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

Keywords

  • Chromosome 19
  • In situ hybridization
  • Myotonic dystrophy
  • Polymorphic DNA linkage markers

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Genetics
  • Developmental Biology
  • Neuroscience(all)

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    Yamaoka, L. H., Bartlett, R. J., Ross, D. A., Fey, G. H., Ledbetter, D. H., Bruns, G., Pericak-Vance, M. A., Herbstreith, M. H., & Roses, A. D. (1985). Localization of cloned unique DNA to three different regions of chromosome 19: Screen for linkage probes for myotonic dystrophy. Journal of Neurogenetics, 2(6), 403-412. https://doi.org/10.3109/01677068509101426