Localization of B-cell stimulatory activity of HIV-1 to the carboxyl terminus of gp41

N. Chirmule, V. S. Kalyanaraman, C. Saxinger, F. Wong-Staal, J. Ghrayeb, Savita G Pahwa

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Patients with AIDS are known to have B-cell hyperactivity. We have previously demonstrated that an extract of HIV-1 could induce differentiation of peripheral blood B lymphocytes of healthy volunteers into immunoglobulin-secreting cells. In an attempt to delineate the B-cell stimulatory subregion in HIV-1, we have investigated the influences of native glycoproteins and recombinant proteins of the envelope. The complete envelope glycoprotein, gp160 and a recombinant protein in the carboxyl terminal region of gp41 termed PE-8 were effective in inducing terminal differentiation of normal peripheral blood B lymphocytes and did so in a T-lymphocyte-dependent manner. The activity was not present in the native exterior envelope glycoprotein, gp120 and several other recombinant proteins, viz PE-2 an PE-3, which are in the amino terminal region of gp120 or in env-9, a protein in the junctional region of gp120 and gp41. Polyclonal and monoclonal antibodies directed to diverse regions of the envelope abrogated the influence of gp160. The PE-8-induced B-cell differentiation was abrogated by goat anti-gp160 antibody but not by goat anti-gp120 antibody or monoclonal antibody to the amino terminal of gp41. These studies suggest that a putative polyclonal B-cell stimulatory epitope of HIV-1 is located in the carboxyl end of the envelope glycoprotein.

Original languageEnglish
Pages (from-to)299-305
Number of pages7
JournalAIDS Research and Human Retroviruses
Volume6
Issue number3
StatePublished - Jan 1 1990
Externally publishedYes

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HIV-1
B-Lymphocytes
Glycoproteins
Recombinant Proteins
Goats
Anti-Idiotypic Antibodies
Monoclonal Antibodies
B-Lymphocyte Epitopes
Antibody-Producing Cells
Cell Differentiation
Healthy Volunteers
Acquired Immunodeficiency Syndrome
T-Lymphocytes
Proteins

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Chirmule, N., Kalyanaraman, V. S., Saxinger, C., Wong-Staal, F., Ghrayeb, J., & Pahwa, S. G. (1990). Localization of B-cell stimulatory activity of HIV-1 to the carboxyl terminus of gp41. AIDS Research and Human Retroviruses, 6(3), 299-305.

Localization of B-cell stimulatory activity of HIV-1 to the carboxyl terminus of gp41. / Chirmule, N.; Kalyanaraman, V. S.; Saxinger, C.; Wong-Staal, F.; Ghrayeb, J.; Pahwa, Savita G.

In: AIDS Research and Human Retroviruses, Vol. 6, No. 3, 01.01.1990, p. 299-305.

Research output: Contribution to journalArticle

Chirmule, N, Kalyanaraman, VS, Saxinger, C, Wong-Staal, F, Ghrayeb, J & Pahwa, SG 1990, 'Localization of B-cell stimulatory activity of HIV-1 to the carboxyl terminus of gp41', AIDS Research and Human Retroviruses, vol. 6, no. 3, pp. 299-305.
Chirmule N, Kalyanaraman VS, Saxinger C, Wong-Staal F, Ghrayeb J, Pahwa SG. Localization of B-cell stimulatory activity of HIV-1 to the carboxyl terminus of gp41. AIDS Research and Human Retroviruses. 1990 Jan 1;6(3):299-305.
Chirmule, N. ; Kalyanaraman, V. S. ; Saxinger, C. ; Wong-Staal, F. ; Ghrayeb, J. ; Pahwa, Savita G. / Localization of B-cell stimulatory activity of HIV-1 to the carboxyl terminus of gp41. In: AIDS Research and Human Retroviruses. 1990 ; Vol. 6, No. 3. pp. 299-305.
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