Abstract
Antibodies to pig lung angiotensin converting enzyme (kininase II) were conjugated to a heme octapeptide (8 microperoxidase, 8 MP) derived from cytochrome c. 8 MP, which has only one reactive amine, was coupled to antibody in a twostep procedure using a bifunctional active ester, bis succinyl succinate. In the first step, 8 MP was reacted with an excess of bis succinyl succinate to yield 8 MP succinyl succinate, a stable compound which can be stored. In a second step the remaining active ester was used for coupling to reactive amines of the antibody. The conjugate consists of 1.6-2.3 8 TP moieties per antibody. Using these procedures, the formation of complex polymers is avoided. Each molecular of conjugate possesses both immunoreactivity and peroxidatic activity. The conjugate has been used to localize angiotensin converting enzyme along the plasma membrane and associated caveolae of pig aortic endothelial cells in culture.
| Original language | English |
|---|---|
| Pages (from-to) | 111-124 |
| Number of pages | 14 |
| Journal | Tissue and Cell |
| Volume | 8 |
| Issue number | 1 |
| DOIs | |
| State | Published - Dec 1 1976 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Insect Science
- Cell Biology
Cite this
Localization of angiotensin converting enzyme (Kininase II). I. Preparation of antibody heme octapeptide conjugates. / Ryan, J. W.; Day, A. R.; Schultz, D. R.; Ryan, U. S.; Chung, A.; Marlborough, D. I.; Dorer, F. E.
In: Tissue and Cell, Vol. 8, No. 1, 01.12.1976, p. 111-124.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Localization of angiotensin converting enzyme (Kininase II). I. Preparation of antibody heme octapeptide conjugates
AU - Ryan, J. W.
AU - Day, A. R.
AU - Schultz, D. R.
AU - Ryan, U. S.
AU - Chung, A.
AU - Marlborough, D. I.
AU - Dorer, F. E.
PY - 1976/12/1
Y1 - 1976/12/1
N2 - Antibodies to pig lung angiotensin converting enzyme (kininase II) were conjugated to a heme octapeptide (8 microperoxidase, 8 MP) derived from cytochrome c. 8 MP, which has only one reactive amine, was coupled to antibody in a twostep procedure using a bifunctional active ester, bis succinyl succinate. In the first step, 8 MP was reacted with an excess of bis succinyl succinate to yield 8 MP succinyl succinate, a stable compound which can be stored. In a second step the remaining active ester was used for coupling to reactive amines of the antibody. The conjugate consists of 1.6-2.3 8 TP moieties per antibody. Using these procedures, the formation of complex polymers is avoided. Each molecular of conjugate possesses both immunoreactivity and peroxidatic activity. The conjugate has been used to localize angiotensin converting enzyme along the plasma membrane and associated caveolae of pig aortic endothelial cells in culture.
AB - Antibodies to pig lung angiotensin converting enzyme (kininase II) were conjugated to a heme octapeptide (8 microperoxidase, 8 MP) derived from cytochrome c. 8 MP, which has only one reactive amine, was coupled to antibody in a twostep procedure using a bifunctional active ester, bis succinyl succinate. In the first step, 8 MP was reacted with an excess of bis succinyl succinate to yield 8 MP succinyl succinate, a stable compound which can be stored. In a second step the remaining active ester was used for coupling to reactive amines of the antibody. The conjugate consists of 1.6-2.3 8 TP moieties per antibody. Using these procedures, the formation of complex polymers is avoided. Each molecular of conjugate possesses both immunoreactivity and peroxidatic activity. The conjugate has been used to localize angiotensin converting enzyme along the plasma membrane and associated caveolae of pig aortic endothelial cells in culture.
UR - http://www.scopus.com/inward/record.url?scp=84886617409&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84886617409&partnerID=8YFLogxK
U2 - 10.1016/0028-2243(76)90082-4
DO - 10.1016/0028-2243(76)90082-4
M3 - Article
C2 - 178067
AN - SCOPUS:84886617409
VL - 8
SP - 111
EP - 124
JO - Tissue and Cell
JF - Tissue and Cell
SN - 0040-8166
IS - 1
ER -