Localization of angiotensin converting enzyme (Kininase II). I. Preparation of antibody heme octapeptide conjugates

Hideki Yotsumoto, Shoichiro Sato, Masabumi Shibuya

Research output: Contribution to journalArticle

Abstract

Antibodies to pig lung angiotensin converting enzyme (kininase II) were conjugated to a heme octapeptide (8 microperoxidase, 8 MP) derived from cytochrome c. 8 MP, which has only one reactive amine, was coupled to antibody in a twostep procedure using a bifunctional active ester, bis succinyl succinate. In the first step, 8 MP was reacted with an excess of bis succinyl succinate to yield 8 MP succinyl succinate, a stable compound which can be stored. In a second step the remaining active ester was used for coupling to reactive amines of the antibody. The conjugate consists of 1.6-2.3 8 TP moieties per antibody. Using these procedures, the formation of complex polymers is avoided. Each molecular of conjugate possesses both immunoreactivity and peroxidatic activity. The conjugate has been used to localize angiotensin converting enzyme along the plasma membrane and associated caveolae of pig aortic endothelial cells in culture.

Original languageEnglish (US)
Pages (from-to)111-124
Number of pages14
JournalUnknown Journal
Volume8
Issue number1
DOIs
StatePublished - 1976

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Localization of angiotensin converting enzyme (Kininase II). I. Preparation of antibody heme octapeptide conjugates'. Together they form a unique fingerprint.

  • Cite this