Local cerebral glucose utilization and cytoskeletal proteolysis as indices of evolving focal ischemic injury in core and penumbra

H. Yao, Myron Ginsberg, D. D. Eveleth, J. C. LaManna, B. D. Watson, O. F. Alonso, J. Y. Loor, J. H. Foreman, R. Busto

Research output: Contribution to journalArticle

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Abstract

To ascertain the tempo of progression to irreversible injury in focal ischemia, we subjected halothane-anesthetized Sprague-Dawley rats to photochemically induced distal middle cerebral artery occlusion (dMCAO) combined with permanent ipsilateral and 1 h contralateral common carotid artery occlusions. Head temperature was maintained at 36°C. At times centered at either 1.5 or 3 h post-dMCAO, the rate of local glucose metabolism (ICMRgl) was measured by 2-deoxyglucose autoradiography, and cytoskeletal proteolysis was assessed regionally by an immunoblotting procedure to detect spectrin breakdown products. At 1.5 h (n = 5), the cortical ischemic core was already severely hypometabolic (ICMRgl 15.5 ± 10.8 μmol 100 g-1 min-1, mean ± SD), whereas the cortical penumbral zone was hypermetabolic (69.0 ± 9.7). (The lumped constant was verified to be unchanged by methylglucose studies.) Neutral red pH studies at this time point showed that both the core and penumbralzones were equally acidotic. By 3 h post-dMCAO (n = 6), ICMRgl in the penumbral zone had fallen to low levels (15.4 ± 2.2 μmol 100 g-1 min-1) equal to those of the ischemic core (16.7 ± 4.5). Correspondingly, spectrin breakdown in the ischemic core was advanced at both 2 and 3.5 h post-dMCAO (36 ± 18% and 33 ± 18% of total spectrin, respectively), whereas in the penumbral zone spectrin breakdown was less extensive and more highly variable at both times (22 ± 23% and 29 ± 16%). We conclude that irreversible deterioration of the ischemic core, as evidenced by the onset of local cytoskeletal proteolysis, begins within 2 h of middle cerebral artery occlusion. In the ischemic penumbra, the transition from glucose hyper- to hypometabolism occurs by 3.5 h and is associated with a milder and more variable degree of spectrin breakdown.

Original languageEnglish
Pages (from-to)398-408
Number of pages11
JournalJournal of Cerebral Blood Flow and Metabolism
Volume15
Issue number3
StatePublished - Jan 1 1995

Fingerprint

Spectrin
Middle Cerebral Artery Infarction
Proteolysis
Glucose
Wounds and Injuries
Neutral Red
Common Carotid Artery
Deoxyglucose
Halothane
Autoradiography
Immunoblotting
Sprague Dawley Rats
Ischemia
Head
Temperature

Keywords

  • 2-Deoxyglucose
  • Calpain
  • Middle cerebral artery occlusion
  • Neutral red
  • Photothrombosis
  • Spectrin

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Neuroscience(all)

Cite this

Local cerebral glucose utilization and cytoskeletal proteolysis as indices of evolving focal ischemic injury in core and penumbra. / Yao, H.; Ginsberg, Myron; Eveleth, D. D.; LaManna, J. C.; Watson, B. D.; Alonso, O. F.; Loor, J. Y.; Foreman, J. H.; Busto, R.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 15, No. 3, 01.01.1995, p. 398-408.

Research output: Contribution to journalArticle

Yao, H, Ginsberg, M, Eveleth, DD, LaManna, JC, Watson, BD, Alonso, OF, Loor, JY, Foreman, JH & Busto, R 1995, 'Local cerebral glucose utilization and cytoskeletal proteolysis as indices of evolving focal ischemic injury in core and penumbra', Journal of Cerebral Blood Flow and Metabolism, vol. 15, no. 3, pp. 398-408.
Yao, H. ; Ginsberg, Myron ; Eveleth, D. D. ; LaManna, J. C. ; Watson, B. D. ; Alonso, O. F. ; Loor, J. Y. ; Foreman, J. H. ; Busto, R. / Local cerebral glucose utilization and cytoskeletal proteolysis as indices of evolving focal ischemic injury in core and penumbra. In: Journal of Cerebral Blood Flow and Metabolism. 1995 ; Vol. 15, No. 3. pp. 398-408.
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abstract = "To ascertain the tempo of progression to irreversible injury in focal ischemia, we subjected halothane-anesthetized Sprague-Dawley rats to photochemically induced distal middle cerebral artery occlusion (dMCAO) combined with permanent ipsilateral and 1 h contralateral common carotid artery occlusions. Head temperature was maintained at 36°C. At times centered at either 1.5 or 3 h post-dMCAO, the rate of local glucose metabolism (ICMRgl) was measured by 2-deoxyglucose autoradiography, and cytoskeletal proteolysis was assessed regionally by an immunoblotting procedure to detect spectrin breakdown products. At 1.5 h (n = 5), the cortical ischemic core was already severely hypometabolic (ICMRgl 15.5 ± 10.8 μmol 100 g-1 min-1, mean ± SD), whereas the cortical penumbral zone was hypermetabolic (69.0 ± 9.7). (The lumped constant was verified to be unchanged by methylglucose studies.) Neutral red pH studies at this time point showed that both the core and penumbralzones were equally acidotic. By 3 h post-dMCAO (n = 6), ICMRgl in the penumbral zone had fallen to low levels (15.4 ± 2.2 μmol 100 g-1 min-1) equal to those of the ischemic core (16.7 ± 4.5). Correspondingly, spectrin breakdown in the ischemic core was advanced at both 2 and 3.5 h post-dMCAO (36 ± 18{\%} and 33 ± 18{\%} of total spectrin, respectively), whereas in the penumbral zone spectrin breakdown was less extensive and more highly variable at both times (22 ± 23{\%} and 29 ± 16{\%}). We conclude that irreversible deterioration of the ischemic core, as evidenced by the onset of local cytoskeletal proteolysis, begins within 2 h of middle cerebral artery occlusion. In the ischemic penumbra, the transition from glucose hyper- to hypometabolism occurs by 3.5 h and is associated with a milder and more variable degree of spectrin breakdown.",
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AU - LaManna, J. C.

AU - Watson, B. D.

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N2 - To ascertain the tempo of progression to irreversible injury in focal ischemia, we subjected halothane-anesthetized Sprague-Dawley rats to photochemically induced distal middle cerebral artery occlusion (dMCAO) combined with permanent ipsilateral and 1 h contralateral common carotid artery occlusions. Head temperature was maintained at 36°C. At times centered at either 1.5 or 3 h post-dMCAO, the rate of local glucose metabolism (ICMRgl) was measured by 2-deoxyglucose autoradiography, and cytoskeletal proteolysis was assessed regionally by an immunoblotting procedure to detect spectrin breakdown products. At 1.5 h (n = 5), the cortical ischemic core was already severely hypometabolic (ICMRgl 15.5 ± 10.8 μmol 100 g-1 min-1, mean ± SD), whereas the cortical penumbral zone was hypermetabolic (69.0 ± 9.7). (The lumped constant was verified to be unchanged by methylglucose studies.) Neutral red pH studies at this time point showed that both the core and penumbralzones were equally acidotic. By 3 h post-dMCAO (n = 6), ICMRgl in the penumbral zone had fallen to low levels (15.4 ± 2.2 μmol 100 g-1 min-1) equal to those of the ischemic core (16.7 ± 4.5). Correspondingly, spectrin breakdown in the ischemic core was advanced at both 2 and 3.5 h post-dMCAO (36 ± 18% and 33 ± 18% of total spectrin, respectively), whereas in the penumbral zone spectrin breakdown was less extensive and more highly variable at both times (22 ± 23% and 29 ± 16%). We conclude that irreversible deterioration of the ischemic core, as evidenced by the onset of local cytoskeletal proteolysis, begins within 2 h of middle cerebral artery occlusion. In the ischemic penumbra, the transition from glucose hyper- to hypometabolism occurs by 3.5 h and is associated with a milder and more variable degree of spectrin breakdown.

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