Local carboplatin therapy in transgenic murine retinoblastoma

J. William Harbour, Timothy G. Murray, Duco Hamasaki, Nicole Cicciarelli, Eleut Hernández, Barbara Smith, Jolene Windle, Joan M. O'Brien

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Abstract

Purpose. To determine the efficacy and toxicity associated with intraocular delivery of carboplatin in the treatment of murine transgenic hereditary retinoblastoma. Methods. Forty-eight transgenic BLH-SV40 Tag retinoblastoma mice were administered five intravitreal injections of carboplatin in one eye. After 12 weeks, the eyes were examined histopathologically to evaluate tumor burden. Twelve rabbits were administered intravitreal injections of carboplatin in one eye, after which they underwent serial electroretinography. All experimental and control eyes were obtained for histopathology and electron microscopy. Results. A dose- dependent inhibition of intraocular tumor growth by carboplatin was observed in transgenic retinoblastoma mice. Tumor development was inhibited in 50% of the mouse eyes at doses of 1.4 μg. In rabbits, retinal toxicity resulted when intravitreal injections of carboplatin were administered at doses of 10 μg or higher. Conclusions. Local delivery of carboplatin in serial doses effectively inhibits intraocular tumor growth in a dose-dependent manner in transgenic murine retinoblastoma.

Original languageEnglish (US)
Pages (from-to)1892-1898
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number9
StatePublished - Aug 1 1996

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Keywords

  • carboplatin
  • chemotherapy
  • retinoblastoma
  • toxicity studies
  • transgenic animal models

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Harbour, J. W., Murray, T. G., Hamasaki, D., Cicciarelli, N., Hernández, E., Smith, B., Windle, J., & O'Brien, J. M. (1996). Local carboplatin therapy in transgenic murine retinoblastoma. Investigative Ophthalmology and Visual Science, 37(9), 1892-1898.