lncRNA RMST Enhances DNMT3 Expression through Interaction with HuR

Wan Xin Peng, Pratirodh Koirala, Wei Zhang, Chao Ni, Zheng Wang, Liu Yang, Yin Yuan Mo

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Large bodies of studies have shown that the CRISPR/Cas9-based library screening is a very powerful tool for the identification of gene functions. However, most of these studies have focused on protein-coding genes, and, furthermore, very few studies have used gene reporters for screening. In the present study, we generated DNA methyltransferase 3B (DNMT3B) reporter and screened a CRISPR/Cas9 synergistic activation mediator (SAM) library against a focused group of lncRNAs. With this screening approach, we identified Rhabdomyosarcoma 2-Associated Transcript (RMST) as a positive regulator for DNMT3B. This was confirmed by activation of the endogenous RMST by SAM or ectopic expression of RMST. Moreover, RMST knockout (KO) suppresses DNMT3, while rescue with RMST in the KO cells restores the DNMT3 level. Finally, RMST KO suppresses global DNA methylation, leading to the upregulation of methylation-regulated genes. Mechanistically, RMST promotes the interaction between the RNA-binding protein HuR and DNMT3B 3′ UTR, increasing the DNMT3B stability. Together, these results not only provide the feasibility of a reporter system for CRISPR library screening but also demonstrate the previously uncharacterized factor RMST as an important player in the modulation of DNA methylation.

Original languageEnglish (US)
JournalMolecular Therapy
DOIs
StateAccepted/In press - Jan 1 2019

Keywords

  • DNA methylation
  • DNMT3
  • HuR
  • RMST
  • lncRNA

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Pharmacology
  • Drug Discovery

Fingerprint Dive into the research topics of 'lncRNA RMST Enhances DNMT3 Expression through Interaction with HuR'. Together they form a unique fingerprint.

  • Cite this

    Peng, W. X., Koirala, P., Zhang, W., Ni, C., Wang, Z., Yang, L., & Mo, Y. Y. (Accepted/In press). lncRNA RMST Enhances DNMT3 Expression through Interaction with HuR. Molecular Therapy. https://doi.org/10.1016/j.ymthe.2019.09.024