Live imaging of multicolor-labeled cells in Drosophila

Maria Boulina, Hasitha Samarajeewa, James D. Baker, Michael D. Kim, Akira Chiba

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

We describe LOLLIbow, a Brainbow-based live imaging system with applications in developmental biology and neurobiology. The development of an animal, including the environmentally sensitive adaptation of its brain, is thought to proceed through continual orchestration among diverse cell types as they divide, migrate, transform and interact with one another within the body. To facilitate direct visualization of such dynamic morphogenesis by individual cells in vivo, we have modified the original Brainbow for Drosophila in which live imaging is practical during much of its development. Our system offers permanent fluorescent labels that reveal fine morphological details of individual cells without requiring dissection or fixation of the samples. It also features a non-invasive means to control the timing of stochastic tricolor cell labeling with a light pulse. We demonstrate applicability of the new system in a variety of settings that could benefit from direct imaging of the developing multicellular organism with single-cell resolution.

Original languageEnglish (US)
Pages (from-to)1605-1613
Number of pages9
JournalDevelopment (Cambridge)
Volume140
Issue number7
DOIs
StatePublished - Apr 1 2013

Keywords

  • Brainbow
  • Drosophila
  • Live imaging
  • Photoactivation
  • Single-cell resolution
  • Split Cre

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

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