Abstract
LITHIUM has neuroprotective effects in a number of model systems which may contribute to the therapeutic effects of lithium in mood disorders. Because the tumor suppressor p53 is linked to cell death, we tested whether lithium administration to human neuroblastoma SHSY5Y cells modulated the activation of p53. After treatment of cells with H7 (25, 50, and 75 μM), nuclear p53 levels were increased to 464, 816 and 1079% of basal levels, respectively. A 24h pretreatment with 5mM lithium reduced these increases by 69, 61 and 28%, respectively. Pretreatment with 2 mM lithium for I or 14 days reduced the 25 μM H7-induced elevations of nuclear p53 by 40 and 70%, respectively, and even a 14-day pretreatment with 1 mM lithium caused a significant 16% reduction. Since increased nuclear p53 is a critical intermediate step in many signaling processes that culminate in cell death, attenuation of p53 activation by lithium reveals a mechanism by which lithium may support neuronal survival.
| Original language | English |
|---|---|
| Pages (from-to) | 1123-1125 |
| Number of pages | 3 |
| Journal | NeuroReport |
| Volume | 10 |
| Issue number | 5 |
| State | Published - Apr 6 1999 |
| Externally published | Yes |
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Keywords
- Apoptosis
- Bipolar disorder
- H7
- Lithium
- Neuroblastoma
- P53
- SH-SY5Y
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Lithium attenuates p53 levels in human neuroblastoma SH-SY5Y cells. / Lu, Rong; Song, Ling; Jope, Richard S.
In: NeuroReport, Vol. 10, No. 5, 06.04.1999, p. 1123-1125.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lithium attenuates p53 levels in human neuroblastoma SH-SY5Y cells
AU - Lu, Rong
AU - Song, Ling
AU - Jope, Richard S
PY - 1999/4/6
Y1 - 1999/4/6
N2 - LITHIUM has neuroprotective effects in a number of model systems which may contribute to the therapeutic effects of lithium in mood disorders. Because the tumor suppressor p53 is linked to cell death, we tested whether lithium administration to human neuroblastoma SHSY5Y cells modulated the activation of p53. After treatment of cells with H7 (25, 50, and 75 μM), nuclear p53 levels were increased to 464, 816 and 1079% of basal levels, respectively. A 24h pretreatment with 5mM lithium reduced these increases by 69, 61 and 28%, respectively. Pretreatment with 2 mM lithium for I or 14 days reduced the 25 μM H7-induced elevations of nuclear p53 by 40 and 70%, respectively, and even a 14-day pretreatment with 1 mM lithium caused a significant 16% reduction. Since increased nuclear p53 is a critical intermediate step in many signaling processes that culminate in cell death, attenuation of p53 activation by lithium reveals a mechanism by which lithium may support neuronal survival.
AB - LITHIUM has neuroprotective effects in a number of model systems which may contribute to the therapeutic effects of lithium in mood disorders. Because the tumor suppressor p53 is linked to cell death, we tested whether lithium administration to human neuroblastoma SHSY5Y cells modulated the activation of p53. After treatment of cells with H7 (25, 50, and 75 μM), nuclear p53 levels were increased to 464, 816 and 1079% of basal levels, respectively. A 24h pretreatment with 5mM lithium reduced these increases by 69, 61 and 28%, respectively. Pretreatment with 2 mM lithium for I or 14 days reduced the 25 μM H7-induced elevations of nuclear p53 by 40 and 70%, respectively, and even a 14-day pretreatment with 1 mM lithium caused a significant 16% reduction. Since increased nuclear p53 is a critical intermediate step in many signaling processes that culminate in cell death, attenuation of p53 activation by lithium reveals a mechanism by which lithium may support neuronal survival.
KW - Apoptosis
KW - Bipolar disorder
KW - H7
KW - Lithium
KW - Neuroblastoma
KW - P53
KW - SH-SY5Y
UR - http://www.scopus.com/inward/record.url?scp=0033528817&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033528817&partnerID=8YFLogxK
M3 - Article
C2 - 10321495
AN - SCOPUS:0033528817
VL - 10
SP - 1123
EP - 1125
JO - NeuroReport
JF - NeuroReport
SN - 0959-4965
IS - 5
ER -