Lipoxygenation of arachidonic acid by differentiated and undifferentiated human promyelocytic HL-60 cells

V. A. Ziboh, T. Wong, M. C. Wu, Adel A Yunis

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The human promyelocytic leukemia cell line HL-60 can be induced to differentiate into mature granulocytes by exposure to dimethyl sulfoxide (DMSO), as well as into cells of monocyte-macrophage lineage by exposure to 12-0-tetradecanoylphorbol-13-acetate (TPA). Incubation of the promyelocytic leukemic HL-60 cells and the two chemically induced differentiated cells with carbon 14-labeled arachidonic acid overnight resulted in the incorporation of label mainly into cellular phospholipids. Stimulation of the labeled cells with Ca2+ ionophore (A23187) resulted in the generation of lipoxygenase products in all the cells. Notably, the DMSO-induced differentiated granulocytic cells biosynthesized predominantly the peptidoeicosatetraenoic acids LTC4 and LTD4 compared with the dihydroxyeicosatetraenoic acid LTB4. On the other hand, the TPA-induced differentiated monocytic-macrophage cells biosynthesized predominantly LTB4 compared with LTC4 and LTD4. The undifferentiated promyelocytic cells lacked the capacity to biosynthesize LTB4, as evidenced in the chemically induced differentiated cells. The monohydroxyeicosatetraenoic acid (HETE) 5-HETE, a metabolite of the 5-lipoxygenase pathway, was a minor component in these cells, and 12-HETE and 15-HETE were barely detectable. These results suggest that the two models of DMSO-induced and TPA-induced differentiated cells may be useful systems for further investigations of arachidonic acid metabolites in granulocytic and monocytic-macrophage functions.

Original languageEnglish (US)
Pages (from-to)161-166
Number of pages6
JournalJournal of Laboratory and Clinical Medicine
Volume108
Issue number2
StatePublished - 1986
Externally publishedYes

Fingerprint

Leukotriene B4
Macrophages
HL-60 Cells
Dimethyl Sulfoxide
Arachidonic Acid
Leukotriene D4
Leukotriene C4
Acetates
Metabolites
Acids
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Arachidonate 5-Lipoxygenase
Lipoxygenase
Ionophores
Tetradecanoylphorbol Acetate
Labels
Phospholipids
Carbon
Cells
Hydroxyeicosatetraenoic Acids

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Lipoxygenation of arachidonic acid by differentiated and undifferentiated human promyelocytic HL-60 cells. / Ziboh, V. A.; Wong, T.; Wu, M. C.; Yunis, Adel A.

In: Journal of Laboratory and Clinical Medicine, Vol. 108, No. 2, 1986, p. 161-166.

Research output: Contribution to journalArticle

Ziboh, V. A. ; Wong, T. ; Wu, M. C. ; Yunis, Adel A. / Lipoxygenation of arachidonic acid by differentiated and undifferentiated human promyelocytic HL-60 cells. In: Journal of Laboratory and Clinical Medicine. 1986 ; Vol. 108, No. 2. pp. 161-166.
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