Liposome-mediated transfer of vascular endothelial growth factor cDNA augments survival of random-pattern skin flaps in the rat

Paul Y. Liu, Wenjing Tong, Kan Liu, Sang Hoon Han, Xiao Tian Wang, Evangelos V Badiavas, Kimberly Rieger-Christ, Ian Summerhayes

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Tissue engineering is an application for gene therapy that is in its infancy. We show that simple liposomal-mediated gene transfer could result in a potentially useful biological effect in the field of wound healing. cDNA encoding the 165 amino acid form of vascular endothelial growth factor complexed to commercially available liposomes was injected into rat skin 1 week before raising a random pattern 3 × 10 cm flap. The flap survival was enhanced by 14 percent, and was accomplished without accessing the arterial inflow of the territory. These results were statistically significant (p < 0.002) and reproducible. No adverse effects were seen. Histological analysis of the angiogenesis localized much of the new vessel formation to the area around the hair follicles. Polymerase chain reaction amplification of extracted flap tissue confirmed the presence of the transgene.

Original languageEnglish
Pages (from-to)80-85
Number of pages6
JournalWound Repair and Regeneration
Volume12
Issue number1
StatePublished - Jan 1 2004
Externally publishedYes

Fingerprint

Hair Follicle
Tissue Engineering
Transgenes
Liposomes
Genetic Therapy
Wound Healing
Vascular Endothelial Growth Factor A
Complementary DNA
Amino Acids
Polymerase Chain Reaction
Skin
Genes

ASJC Scopus subject areas

  • Dermatology
  • Surgery

Cite this

Liposome-mediated transfer of vascular endothelial growth factor cDNA augments survival of random-pattern skin flaps in the rat. / Liu, Paul Y.; Tong, Wenjing; Liu, Kan; Han, Sang Hoon; Wang, Xiao Tian; Badiavas, Evangelos V; Rieger-Christ, Kimberly; Summerhayes, Ian.

In: Wound Repair and Regeneration, Vol. 12, No. 1, 01.01.2004, p. 80-85.

Research output: Contribution to journalArticle

Liu, PY, Tong, W, Liu, K, Han, SH, Wang, XT, Badiavas, EV, Rieger-Christ, K & Summerhayes, I 2004, 'Liposome-mediated transfer of vascular endothelial growth factor cDNA augments survival of random-pattern skin flaps in the rat', Wound Repair and Regeneration, vol. 12, no. 1, pp. 80-85.
Liu, Paul Y. ; Tong, Wenjing ; Liu, Kan ; Han, Sang Hoon ; Wang, Xiao Tian ; Badiavas, Evangelos V ; Rieger-Christ, Kimberly ; Summerhayes, Ian. / Liposome-mediated transfer of vascular endothelial growth factor cDNA augments survival of random-pattern skin flaps in the rat. In: Wound Repair and Regeneration. 2004 ; Vol. 12, No. 1. pp. 80-85.
@article{2f15f448697d4036825f27135a553ec9,
title = "Liposome-mediated transfer of vascular endothelial growth factor cDNA augments survival of random-pattern skin flaps in the rat",
abstract = "Tissue engineering is an application for gene therapy that is in its infancy. We show that simple liposomal-mediated gene transfer could result in a potentially useful biological effect in the field of wound healing. cDNA encoding the 165 amino acid form of vascular endothelial growth factor complexed to commercially available liposomes was injected into rat skin 1 week before raising a random pattern 3 × 10 cm flap. The flap survival was enhanced by 14 percent, and was accomplished without accessing the arterial inflow of the territory. These results were statistically significant (p < 0.002) and reproducible. No adverse effects were seen. Histological analysis of the angiogenesis localized much of the new vessel formation to the area around the hair follicles. Polymerase chain reaction amplification of extracted flap tissue confirmed the presence of the transgene.",
author = "Liu, {Paul Y.} and Wenjing Tong and Kan Liu and Han, {Sang Hoon} and Wang, {Xiao Tian} and Badiavas, {Evangelos V} and Kimberly Rieger-Christ and Ian Summerhayes",
year = "2004",
month = "1",
day = "1",
language = "English",
volume = "12",
pages = "80--85",
journal = "Wound Repair and Regeneration",
issn = "1067-1927",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Liposome-mediated transfer of vascular endothelial growth factor cDNA augments survival of random-pattern skin flaps in the rat

AU - Liu, Paul Y.

AU - Tong, Wenjing

AU - Liu, Kan

AU - Han, Sang Hoon

AU - Wang, Xiao Tian

AU - Badiavas, Evangelos V

AU - Rieger-Christ, Kimberly

AU - Summerhayes, Ian

PY - 2004/1/1

Y1 - 2004/1/1

N2 - Tissue engineering is an application for gene therapy that is in its infancy. We show that simple liposomal-mediated gene transfer could result in a potentially useful biological effect in the field of wound healing. cDNA encoding the 165 amino acid form of vascular endothelial growth factor complexed to commercially available liposomes was injected into rat skin 1 week before raising a random pattern 3 × 10 cm flap. The flap survival was enhanced by 14 percent, and was accomplished without accessing the arterial inflow of the territory. These results were statistically significant (p < 0.002) and reproducible. No adverse effects were seen. Histological analysis of the angiogenesis localized much of the new vessel formation to the area around the hair follicles. Polymerase chain reaction amplification of extracted flap tissue confirmed the presence of the transgene.

AB - Tissue engineering is an application for gene therapy that is in its infancy. We show that simple liposomal-mediated gene transfer could result in a potentially useful biological effect in the field of wound healing. cDNA encoding the 165 amino acid form of vascular endothelial growth factor complexed to commercially available liposomes was injected into rat skin 1 week before raising a random pattern 3 × 10 cm flap. The flap survival was enhanced by 14 percent, and was accomplished without accessing the arterial inflow of the territory. These results were statistically significant (p < 0.002) and reproducible. No adverse effects were seen. Histological analysis of the angiogenesis localized much of the new vessel formation to the area around the hair follicles. Polymerase chain reaction amplification of extracted flap tissue confirmed the presence of the transgene.

UR - http://www.scopus.com/inward/record.url?scp=1342302945&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1342302945&partnerID=8YFLogxK

M3 - Article

C2 - 14974968

AN - SCOPUS:1342302945

VL - 12

SP - 80

EP - 85

JO - Wound Repair and Regeneration

JF - Wound Repair and Regeneration

SN - 1067-1927

IS - 1

ER -