Lipidomics dataset of Danio rerio optic nerve regeneration model

Jennifer Arcuri, Matthew B. Veldman, Sanjoy K. Bhattacharya

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The right optic nerve of adult, 6 month to 1 year old, female and male Danio rerio were crushed and collected three days after. Matching controls of uninjured left optic nerves were also collected. The tissue was dissected from euthanized fish and frozen on dry ice. Samples were pooled for each category (female crush, female control, male crush, male control) n = 24 to obtain sufficient tissue for analysis. The brain from one male fish was also collected for control/calibration. Lipid extraction was done with the Bligh and Dyer [1] method, followed by untargeted liquid chromatography-mass spectrometry (LC MS-MS) lipid profiling using a Q-Exactive Orbitrap instrument coupled with Vanquish Horizon Binary UHPLC LC-MS system. The lipids were identified and quantified with LipidSearch 4.2.21 and the statistical analysis was conducted through Metaboanalyst 5.0. This data is available at Metabolomics Workbench, Study ID ST001725.

Original languageEnglish (US)
Article number107260
JournalData in Brief
Volume37
DOIs
StatePublished - Aug 2021

Keywords

  • CNS Injury
  • Lipids
  • Optic Nerve
  • Regeneration
  • Zebra-fish

ASJC Scopus subject areas

  • General

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