Lipid peroxidation in vivo induced by reversible global ischemia in rat brain

B. D. Watson, R. Busto, W. J. Goldberg, M. Santiso, S. Yoshida, Myron Ginsberg

Research output: Contribution to journalArticle

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Abstract

It has been hypothesized that ischemia, followed by reperfusion, facilitates peroxidative free-radical chain processes in brain. To resolve this question, rats were subjected to reversible global ischemia. From coronal sections of brains frodubl zoen in situ, small (ca. 2 mg) amounts of tissue were sampled from neocortex, hippocampus, and thalamus of both cerebral hemispheres of four groups of rats exposed to 30 min cerebral ischemia followed 0, 30, 60, and 240 min of reperfusion, and from a control group subjected to the same operative procedures, except for the induction of ischemia. Heptane-solubilized total lipid extracts from these sampes were analyzed spectroscopically in the 19--330 nm range for content of isolated (nonconjugated) duble bonds and of conjugated diene structures; the latter are formed from isolated double bonds during peroxidation of unsaturated fatty acids. Spectra derived from tissue regions of rats subjected to ischemia, or ischemia followed by reperfusion, were compared to averaged, region-specific control spectra and were normalized to the original content of isolated double bonds in the peroxidized samples. The resultant difference spectra were analyzed in terms of ratios of conjugated diene concentration to the concentration of isolated double bonds originally at risk in the specific tissue zones considered. The peak representing conjugated diene formation was centered at 238 ± 1 nm and was usually well resolved when the molar ratio [conjugated diene]/[isolated double bonds], expressed as a percentage ([CD/IDB]), was greater than 0.25%. The incidence of resolvable conjugated diene peaks was much greater in the reperfused groups (18 of 124 samples total; 0.25% ≤ [CD]/IDB] ≤ 1.34%, compared to the nonreperfused group (1 of 38 samples; [CD]/IDB] = 0.62%. No specific regional susceptibility to conjugated diene formation was observed. It also was shown by computer averaging over all the tissue zones sampled that, if one had used large samples consisting of pooled brain tissue for analysis of lipid peroxides and/or their reaction products, the conjugated diene peak would have been attenuated below distinctly resolvable levels. These results constitute the first demonstration of lipid peroxidation induced by reversible global ischemia. The observation that the effect is highly focal and not generalized throughout the brain suggests that special conditions of reperfusibility and reoxygenation must be satisfied for lipid peroxidation to be detectable in an ischemic milieu.

Original languageEnglish
Pages (from-to)268-274
Number of pages7
JournalJournal of Neurochemistry
Volume42
Issue number1
StatePublished - Mar 1 1984

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Lipid Peroxidation
Rats
Brain
Ischemia
Tissue
Lipids
Reperfusion
Heptanes
Lipid Peroxides
Unsaturated Fatty Acids
Reaction products
Neocortex
Operative Surgical Procedures
Cerebrum
Free Radicals
Brain Ischemia
Thalamus
Demonstrations
Hippocampus
Control Groups

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Watson, B. D., Busto, R., Goldberg, W. J., Santiso, M., Yoshida, S., & Ginsberg, M. (1984). Lipid peroxidation in vivo induced by reversible global ischemia in rat brain. Journal of Neurochemistry, 42(1), 268-274.

Lipid peroxidation in vivo induced by reversible global ischemia in rat brain. / Watson, B. D.; Busto, R.; Goldberg, W. J.; Santiso, M.; Yoshida, S.; Ginsberg, Myron.

In: Journal of Neurochemistry, Vol. 42, No. 1, 01.03.1984, p. 268-274.

Research output: Contribution to journalArticle

Watson, BD, Busto, R, Goldberg, WJ, Santiso, M, Yoshida, S & Ginsberg, M 1984, 'Lipid peroxidation in vivo induced by reversible global ischemia in rat brain', Journal of Neurochemistry, vol. 42, no. 1, pp. 268-274.
Watson BD, Busto R, Goldberg WJ, Santiso M, Yoshida S, Ginsberg M. Lipid peroxidation in vivo induced by reversible global ischemia in rat brain. Journal of Neurochemistry. 1984 Mar 1;42(1):268-274.
Watson, B. D. ; Busto, R. ; Goldberg, W. J. ; Santiso, M. ; Yoshida, S. ; Ginsberg, Myron. / Lipid peroxidation in vivo induced by reversible global ischemia in rat brain. In: Journal of Neurochemistry. 1984 ; Vol. 42, No. 1. pp. 268-274.
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