Lipid oxidation fuels recovery from exhaustive exercise in white muscle of rainbow trout

Jeff G. Richards, George J F Heigenhauser, Chris M. Wood

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The oxidative utilization of lipid and carbohydrate was examined in white muscle of rainbow trout (Oncorhynchus mykiss) at rest, immediately after exhaustive exercise, and for 32-h recovery. In addition to creatine phosphate and glycolysis fueling exhaustive exercise, near maximal activation of pyruvate dehydrogenase (PDH) at the end of exercise points to oxidative phosphorylation of carbohydrate as an additional source of ATP during exercise. Within 15 min postexercise, PDH activation returned to resting values, thus sparing accumulated lactate from oxidation. Glycogen synthase activity matched the rate of glycogen resynthesis and represented near maximal activation. Decreases in white muscle free carnitine, increases in long-chain fatty acyl carnitine, and sustained elevations of acetyl-CoA and acetyl carnitine indicate a rapid utilization of lipid to supply ATP for recovery. Increases in malonyl-CoA during recovery suggest that malonyl-CoA may not regulate carnitine palmitoyltransferase-1 in trout muscle during recovery, but instead it may act to elongate short-chain fatty acids for mitochondrial oxidation. In addition, decreases in intramuscular triacylglycerol and in plasma nonesterified fatty acids indicate that both endogenous and exogenous lipid fuels may be oxidized during recovery.

Original languageEnglish
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume282
Issue number1 51-1
StatePublished - Jul 2 2002

Fingerprint

Oncorhynchus mykiss
Malonyl Coenzyme A
Carnitine
Pyruvic Acid
Lipids
Muscles
Oxidoreductases
Adenosine Triphosphate
Carbohydrates
Acetylcarnitine
Carnitine O-Palmitoyltransferase
Glycogen Synthase
Acetyl Coenzyme A
Phosphocreatine
Volatile Fatty Acids
Trout
Oxidative Phosphorylation
Glycolysis
Glycogen
Nonesterified Fatty Acids

Keywords

  • Carbohydrate
  • Glycogen synthase
  • Lactate
  • Malonyl-coenzyme A
  • Metabolism
  • Nonesterified fatty acids
  • Pyruvate dehydrogenase

ASJC Scopus subject areas

  • Physiology

Cite this

Lipid oxidation fuels recovery from exhaustive exercise in white muscle of rainbow trout. / Richards, Jeff G.; Heigenhauser, George J F; Wood, Chris M.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 282, No. 1 51-1, 02.07.2002.

Research output: Contribution to journalArticle

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