Linoleic acid potentiates tnf-mediated apoptosis of cultured endothelial cells

Michal J Toborek, E. M. Blanc, S. Aziz, S. Young, M. P. Mattson, B. Hennig

Research output: Contribution to journalArticle

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Abstract

Selected diet-derived lipids may influence cytokine-mediated endothelial cell dysfunction, including TNF-induced apoptosis. To test this hypothesis, oxidative stress, intracellular calcium levels, barrier function, mitochondrial function, as well as chromatin condensation and DNA fragmentation were measured in endothelial cells treated with linolelc acid (18:2; 90 μM) and/or TNF (100 U/mL). In cells exposed to 18:2 plus TNF, pretreatment with 18:2 began 4.5 hours before exposure to TNF. The combined exposure to 18:2 plus TNF was then continued for up to 19.5 hours. After treatment exposure, endothelial cultures were washed and incubated with maintenance medium for up to 7 days. Although initial treatment with TNF or 18:2 significantly increased oxidative stress and intracellular calcium levels, only exposure to TNF induced apoptotic processes in cultured endothelial cells. Furthermore, the combined exposure to 18:2 plus TNF amplified TNF-induced apoptosis. Vitamin E did not protect against TNF-induced apoptosis. However, vitamin E reversed the 18:2-mediated potentiation of apoptosis in endothelial cells exposed to 18:2 plus TNF. These studies provide evidence that the lipid environment may be crucial during local TNF-induced events in the vascular endothelium.

Original languageEnglish
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996
Externally publishedYes

Fingerprint

Endothelial cells
Linoleic Acid
cultured cells
endothelial cells
linoleic acid
Cultured Cells
apoptosis
Endothelial Cells
Apoptosis
Oxidative stress
Vitamin E
vitamin E
Oxidative Stress
oxidative stress
Calcium
Lipids
calcium
DNA fragmentation
Vascular Endothelium
DNA Fragmentation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Toborek, M. J., Blanc, E. M., Aziz, S., Young, S., Mattson, M. P., & Hennig, B. (1996). Linoleic acid potentiates tnf-mediated apoptosis of cultured endothelial cells. FASEB Journal, 10(3).

Linoleic acid potentiates tnf-mediated apoptosis of cultured endothelial cells. / Toborek, Michal J; Blanc, E. M.; Aziz, S.; Young, S.; Mattson, M. P.; Hennig, B.

In: FASEB Journal, Vol. 10, No. 3, 01.12.1996.

Research output: Contribution to journalArticle

Toborek, MJ, Blanc, EM, Aziz, S, Young, S, Mattson, MP & Hennig, B 1996, 'Linoleic acid potentiates tnf-mediated apoptosis of cultured endothelial cells', FASEB Journal, vol. 10, no. 3.
Toborek MJ, Blanc EM, Aziz S, Young S, Mattson MP, Hennig B. Linoleic acid potentiates tnf-mediated apoptosis of cultured endothelial cells. FASEB Journal. 1996 Dec 1;10(3).
Toborek, Michal J ; Blanc, E. M. ; Aziz, S. ; Young, S. ; Mattson, M. P. ; Hennig, B. / Linoleic acid potentiates tnf-mediated apoptosis of cultured endothelial cells. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
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