Linoleic acid amplifies PCB-mediated dysfunction of endothelial cells

R. Slim, Michal J Toborek, L. W. Robertson, B. Hennig

Research output: Contribution to journalArticle

Abstract

Selected dietary lipids may increase the atherogenicity of environmental chemicals, such as polychlorinated biphenyls (PCB), by cross-amplifying mechanisms leading to dysfunction of the vascular endothelium. To investigate this hypothesis, cultured endothelial cells were treated with linoleic acid (18:2), followed by different PCB for up to 18 hours. Only PCB with Ah receptor binding activity disrupted endothelial barrier function by allowing an increase in albumin transfer across endothelial monolayers. Prior cellular enrichment with 18:2 before PCB treatment further diminished endothelial barrier function, as compared to cells treated only with PCB. This phenomenon appears to be mediated by increased oxidative stress, which can be supported by enhanced DCF fluorescence, activation of NF-κB, as well as an observed decrease in vitamin E content in the culture media. Similar to the endothelial permeability data, pre-enrichment of cells with 18:2 further increased the PCB-mediated activity and content of cytochrome P450 1A. Our results suggest that certain unsaturated fatty acids can potentiate PCB-mediated endothelial cell dysfunction and that oxidative stress and activation of the cytochrome P450 1A subfamily may be in part responsible for these metabolic events. These findings have implications for understanding the involvement of certain environmental contaminants in diseases that involve dysfunction of the vascular endothelium.

Original languageEnglish
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998
Externally publishedYes

Fingerprint

Polychlorinated Biphenyls
Endothelial cells
polychlorinated biphenyls
Linoleic Acid
endothelial cells
linoleic acid
Endothelial Cells
Oxidative stress
Vascular Endothelium
endothelium
blood vessels
cytochrome P-450
Cytochrome P-450 Enzyme System
Oxidative Stress
oxidative stress
Chemical activation
Unsaturated Fatty Acids
Vitamin E
unsaturated fatty acids
dietary fat

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Linoleic acid amplifies PCB-mediated dysfunction of endothelial cells. / Slim, R.; Toborek, Michal J; Robertson, L. W.; Hennig, B.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

Slim, R. ; Toborek, Michal J ; Robertson, L. W. ; Hennig, B. / Linoleic acid amplifies PCB-mediated dysfunction of endothelial cells. In: FASEB Journal. 1998 ; Vol. 12, No. 5.
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