Limitations of tetrazolium salts in delineating infarcted brain

T. M. Liszczak, E. T. Hedley-Whyte, J. F. Adams, D. H. Han, V. S. Kolluri, F. X. Vacanti, Roberto Heros, N. T. Zervas

Research output: Contribution to journalArticle

150 Citations (Scopus)

Abstract

Tetrazolium salts, histochemical indicators of mitochondrial respiratory enzymes, have been used by some pathologists to detect infarcts in myocardium. We explored the utility of this technique in detecting experimental brain infarcts and report our findings. Infarcts were produced in cats, gerbils, and rats by unilateral temporal and permanent cerebral vessel occlusion. After various time periods the animals were killed, and their brains were reacted with 2,3,5, triphenyl, 2H-tetrazolium chloride (TTC). The experimental and contralateral hemispheres were examined by light and electron microscopy. The TTC-stained tissue was correlated with histology. In some situations the histological condition of the tissue correlated well with the TTC staining results. Brain regions supplied by temporarily occluded vessels and judged infarcted by light and electron microscopy did not stain. In these regions less than 6% of the mitochondria were intact. In brain tissue from animals with permanent vessel occlusion (no reflow) mitochondria were intact despite the fact that other cellular organelles, such as nuclei, were destroyed. TTC stained such mitochondria and as a result could not distinguish infarcted brain in complete ischemia situations (no reflow). Another draw back to this staining procedure was 36 h after infarction macrophages with intact mitochondria would replace damage neurons and be stained. Under ideal conditions though this technique can detect irreversibly damaged brain as early as 2.5 h after artery occlusion.

Original languageEnglish
Pages (from-to)150-157
Number of pages8
JournalActa Neuropathologica
Volume65
Issue number2
DOIs
StatePublished - Jun 1 1984
Externally publishedYes

Fingerprint

Tetrazolium Salts
Mitochondria
Brain
Chlorides
Electron Microscopy
Staining and Labeling
Light
Gerbillinae
Organelles
Infarction
Myocardium
Histology
Cats
Coloring Agents
Ischemia
Arteries
Macrophages
Neurons
Enzymes

Keywords

  • Brain
  • Delineation
  • Histochemical
  • Infarcted

ASJC Scopus subject areas

  • Neuroscience(all)
  • Pathology and Forensic Medicine
  • Clinical Neurology

Cite this

Liszczak, T. M., Hedley-Whyte, E. T., Adams, J. F., Han, D. H., Kolluri, V. S., Vacanti, F. X., ... Zervas, N. T. (1984). Limitations of tetrazolium salts in delineating infarcted brain. Acta Neuropathologica, 65(2), 150-157. https://doi.org/10.1007/BF00690469

Limitations of tetrazolium salts in delineating infarcted brain. / Liszczak, T. M.; Hedley-Whyte, E. T.; Adams, J. F.; Han, D. H.; Kolluri, V. S.; Vacanti, F. X.; Heros, Roberto; Zervas, N. T.

In: Acta Neuropathologica, Vol. 65, No. 2, 01.06.1984, p. 150-157.

Research output: Contribution to journalArticle

Liszczak, TM, Hedley-Whyte, ET, Adams, JF, Han, DH, Kolluri, VS, Vacanti, FX, Heros, R & Zervas, NT 1984, 'Limitations of tetrazolium salts in delineating infarcted brain', Acta Neuropathologica, vol. 65, no. 2, pp. 150-157. https://doi.org/10.1007/BF00690469
Liszczak TM, Hedley-Whyte ET, Adams JF, Han DH, Kolluri VS, Vacanti FX et al. Limitations of tetrazolium salts in delineating infarcted brain. Acta Neuropathologica. 1984 Jun 1;65(2):150-157. https://doi.org/10.1007/BF00690469
Liszczak, T. M. ; Hedley-Whyte, E. T. ; Adams, J. F. ; Han, D. H. ; Kolluri, V. S. ; Vacanti, F. X. ; Heros, Roberto ; Zervas, N. T. / Limitations of tetrazolium salts in delineating infarcted brain. In: Acta Neuropathologica. 1984 ; Vol. 65, No. 2. pp. 150-157.
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