Light scattering as an intrinsic indicator for pancreatic islet cell mass and secretion

E. Ilegems, P. P. Van Krieken, P. K. Edlund, A. Dicker, T. Alanentalo, M. Eriksson, S. Mandic, U. Ahlgren, P. O. Berggren

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The pancreatic islet of Langerhans is composed of endocrine cells producing and releasing hormones from secretory granules in response to various stimuli for maintenance of blood glucose homeostasis. In order to adapt to a variation in functional demands, these islets are capable of modulating their hormone secretion by increasing the number of endocrine cells as well as the functional response of individual cells. A failure in adaptive mechanisms will lead to inadequate blood glucose regulation and thereby to the development of diabetes. It is therefore necessary to develop tools for the assessment of both pancreatic islet mass and function, with the aim of understanding cellular regulatory mechanisms and factors guiding islet plasticity. Although most of the existing techniques rely on the use of artificial indicators, we present an imaging methodology based on intrinsic optical properties originating from mature insulin secretory granules within endocrine cells that reveals both pancreatic islet mass and function. We demonstrate the advantage of using this imaging strategy by monitoring in vivo scattering signal from pancreatic islets engrafted into the anterior chamber of the mouse eye, and how this versatile and noninvasive methodology permits the characterization of islet morphology and plasticity as well as hormone secretory status.

Original languageEnglish (US)
Article number10740
JournalScientific Reports
Volume5
DOIs
StatePublished - Jun 1 2015
Externally publishedYes

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Islets of Langerhans
Endocrine Cells
Light
Secretory Vesicles
Hormones
Blood Glucose
Anterior Chamber
Homeostasis
Maintenance
Insulin

ASJC Scopus subject areas

  • General

Cite this

Ilegems, E., Van Krieken, P. P., Edlund, P. K., Dicker, A., Alanentalo, T., Eriksson, M., ... Berggren, P. O. (2015). Light scattering as an intrinsic indicator for pancreatic islet cell mass and secretion. Scientific Reports, 5, [10740]. https://doi.org/10.1038/srep10740

Light scattering as an intrinsic indicator for pancreatic islet cell mass and secretion. / Ilegems, E.; Van Krieken, P. P.; Edlund, P. K.; Dicker, A.; Alanentalo, T.; Eriksson, M.; Mandic, S.; Ahlgren, U.; Berggren, P. O.

In: Scientific Reports, Vol. 5, 10740, 01.06.2015.

Research output: Contribution to journalArticle

Ilegems, E, Van Krieken, PP, Edlund, PK, Dicker, A, Alanentalo, T, Eriksson, M, Mandic, S, Ahlgren, U & Berggren, PO 2015, 'Light scattering as an intrinsic indicator for pancreatic islet cell mass and secretion', Scientific Reports, vol. 5, 10740. https://doi.org/10.1038/srep10740
Ilegems E, Van Krieken PP, Edlund PK, Dicker A, Alanentalo T, Eriksson M et al. Light scattering as an intrinsic indicator for pancreatic islet cell mass and secretion. Scientific Reports. 2015 Jun 1;5. 10740. https://doi.org/10.1038/srep10740
Ilegems, E. ; Van Krieken, P. P. ; Edlund, P. K. ; Dicker, A. ; Alanentalo, T. ; Eriksson, M. ; Mandic, S. ; Ahlgren, U. ; Berggren, P. O. / Light scattering as an intrinsic indicator for pancreatic islet cell mass and secretion. In: Scientific Reports. 2015 ; Vol. 5.
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