Ligand-modified aminobisphosphonate for linking proteins to hydroxyapatite and bone surface

Robin S. Ehrick, Marcello Capaccio, David A. Puleo, Leonidas G Bachas

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

An increase in bone resorption is one of the main symptoms of osteoporosis, a disease that affects more and more individuals every day. Bisphosphonates are known to inhibit bone resorption and thus are being used as a treatment for osteoporosis. Aminobisphosphonates present a functionality that can be easily used for conjugation to other molecules, such as peptides, proteins, and ligands for protein recognition. In this study, an aminobisphosphonate conjugated with biotin was used as a model linker for protein attachment to bone. With this system, the interaction of biotinylated aminobisphosphonate with hydroxyapatite, a major mineral component of bone, was investigated. Quantification of the binding of aminobisphosphonate to hydroxyapatite was performed using a fluorescently labeled antibody for biotin. Additionally, the interaction of the biotinylated aminobisphosphonate with multiple treatments of cortical bone from the midshaft of a cow femur was studied. It was demonstrated that modified aminobisphosphonate reagents can bind hydroxyapatite and bone at high levels, while the biotin functionality is free to be recognized by the fluorescently labeled antibiotin antibody, suggesting that modified aminobisphosphonates could be used to link other peptides or proteins to the bone surface.

Original languageEnglish (US)
Pages (from-to)315-321
Number of pages7
JournalBioconjugate Chemistry
Volume19
Issue number1
DOIs
StatePublished - Jan 2008
Externally publishedYes

Fingerprint

Durapatite
Hydroxyapatite
Bone
Biotin
Ligands
Proteins
Bone and Bones
Bone Resorption
Osteoporosis
Peptides
Antibodies
Diphosphonates
Femur
Minerals
Molecules

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Clinical Biochemistry
  • Chemistry(all)
  • Organic Chemistry

Cite this

Ligand-modified aminobisphosphonate for linking proteins to hydroxyapatite and bone surface. / Ehrick, Robin S.; Capaccio, Marcello; Puleo, David A.; Bachas, Leonidas G.

In: Bioconjugate Chemistry, Vol. 19, No. 1, 01.2008, p. 315-321.

Research output: Contribution to journalArticle

Ehrick, Robin S. ; Capaccio, Marcello ; Puleo, David A. ; Bachas, Leonidas G. / Ligand-modified aminobisphosphonate for linking proteins to hydroxyapatite and bone surface. In: Bioconjugate Chemistry. 2008 ; Vol. 19, No. 1. pp. 315-321.
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