With the use of rabbit anti LH RH serum, a system of delicate nerve fibers and terminals which contain immunoreactive LH RH can be demonstrated in the median eminence of the rat, corresponding exactly to the tuberoinfundibular tract as described earlier with the aid of classical neurohistological methods. The delicate fiber system can be traced to the region of the retrochiasmatic area, and especially to that of the arcuate nucleus, but not farther. Nerve cells do not show any immunopositive reaction for LH RH in the experimental conditions studied. LH RH positive nerve fibers terminate on or in the immediate vicinity of the capillary loops penetrating the median eminence.
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