Levels of E2A protein expression in B cell precursors are stage-dependent and inhibited by stem cell factor (c-kit ligand)

Richard L Riley, Jennean Knowles, Anne M. King

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Abstract

Objective. The E2A-encoded proteins E47 and E12 are crucial to the development of pro-B and pre-B cells. The expression of E2A protein and mRNA during early B lymphopoiesis was determined and effects of stem cell factor (SCF; Steel factor; c-kit ligand) on E2A expression were evaluated. Materials and Methods. Ex vivo murine pro-B cells and pre-B cells were isolated and in vitro B cell precursors were derived after culture of bone marrow with rmIL-7. Levels of E2A proteins were determined by Western analysis and mRNA by RT-PCR. E2A expression in vitro was also assessed in cultures supplemented with IL-7 ± recombinant murine SCF (rmSCF), insulin-like growth factor-1 (rhIGF-1), or Flt3-ligand (rhFlt3-L). Turnover of E2A proteins was assessed following cycloheximide treatment. Results. Ex vivo, pro-B cells had lower E47 protein levels than did pre-B cells but had comparable E2A mRNA levels. E2A protein, but not mRNA, levels were reduced in pro-B cells upon culture in vitro with IL-7 + rmSCF. This was associated with increased turnover of E2A proteins. In contrast, culture with IL-7 + IGF-1 or Flt3-L had minimal effects on E2A protein levels. Conclusion. Pre-B cells expressed higher levels of E2A protein than did pro-B cells and this mainly resulted from posttranscriptional regulation. Exogenous SCF inhibited E2A protein, but not mRNA, expression in cultured B cell precursors, possibly by increasing E2A protein turnover. The capacity to respond to SCF may influence the levels of E2A during B-cell development.

Original languageEnglish
Pages (from-to)1412-1418
Number of pages7
JournalExperimental Hematology
Volume30
Issue number12
DOIs
StatePublished - Dec 1 2002

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ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

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