Leukocyte trafficking to sites of cutaneous exanthem in SIV - Infected macaques

V. Sasseville, J. Rottman, Z. Du, Ronald Charles Desrosiers, A. Lackner

Research output: Contribution to journalArticle

Abstract

HIV exanthem is a common syndrome arising prior to seroconversion to HIV. A similar rash is seen in macaques infected with most isolates of SIV. HIV/SIV exanthem are associated with high levels of viremia, but virus is not seen in skin lesions. However, we have shown that molecular clones of SIVmac239 with a single amino acid substitution in nef cause an exaggerated acute disease syndrome including rash. Biopsies obtained throughout the course of clinically evident rash were examined for the presence of virus and the cellular infiltrate was characterized with respect to cellular immunophenotype. The onset of rash is earlier than that observed with other strains of SIV and is associated with cellular infiltrates containing abundant SIV nucleic acid and protein. Cellular infiltrates within skin biopsies, obtained on the day of rash onset, contain equal numbers of CD4+ and CD8+ lymphocytes and abundant aEb7 positive cells surrounding vessels with upregulated endothelial E-selectin, which is compatible with active recruitment of memory T cells to the skin. By examining sequential skin biopsies from the same animal, we show that clearance of virus and resolution of rash is associated with an increase in the percentage of perivascular inflammatory cells expressing CD8, the chemokine receptor CXCR3 and GMP-17, a marker of cytotoxic granules. These results suggest that activated cytoloxic T cells are trafficking to sites of inflammation in the skin and directly or indirectly affect levels of viral replication at these sites.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998
Externally publishedYes

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ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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