Abstract
The Transfusion Safety Study (TSS) is a multicenter, cooperative investigation of factors that may determine the occurrence and modify the expression of transusion-transmitted infections. A flow cytometry laboratory was established in each of the six participating centers in order to avoid alterations in cell phenotypes which may be caused by shipping delays, temperature changes, and handling. As a consequence, in order to assure compatibility of results, stringent standardization, quality control, and proficiency testing procedures were developed. This paper documents (i) the effect of time from phlebotomy to specimen staining and then to analysis for the antibodies used in the study; (ii) the effects of variations in light scatter cursor location for certain antibodies; (iii) a quality control program and data management and analysis system, each specifically designed for the study; and (iv) presents extensive data on age- and sex-related reference (normal) ranges for the several individual and paired monoclonal antibodies used in the study. Problems encountered, including obtaining reliable absolute lymphocyte counts, interference by nucleated erythrocytes, and sources of variability in results, are discussed. This study is meant to serve as a reference for future TSS publications.
| Original language | English |
|---|---|
| Pages (from-to) | 187-220 |
| Number of pages | 34 |
| Journal | Clinical Immunology and Immunopathology |
| Volume | 55 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 1990 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
- Pathology and Forensic Medicine
Cite this
Leukocyte immunophenotyping by flow cytometry in a multisite study : Standardization, quality control, and normal values in the Transfusion Safety Study. / Parker, John W.; Adelsberg, Bernard; Azen, Stanley P.; Boone, Donna; Fletcher, Mary Ann; Gjerset, George F.; Hassett, Joseph; Kaplan, Joseph; Niland, Joyce C.; Odom-Maryon, Tamara; Operskalski, Eva A.; Prince, Harry; Scott, Diane; Stites, Daniel P.; Mosley, James W.
In: Clinical Immunology and Immunopathology, Vol. 55, No. 2, 01.01.1990, p. 187-220.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Leukocyte immunophenotyping by flow cytometry in a multisite study
T2 - Standardization, quality control, and normal values in the Transfusion Safety Study
AU - Parker, John W.
AU - Adelsberg, Bernard
AU - Azen, Stanley P.
AU - Boone, Donna
AU - Fletcher, Mary Ann
AU - Gjerset, George F.
AU - Hassett, Joseph
AU - Kaplan, Joseph
AU - Niland, Joyce C.
AU - Odom-Maryon, Tamara
AU - Operskalski, Eva A.
AU - Prince, Harry
AU - Scott, Diane
AU - Stites, Daniel P.
AU - Mosley, James W.
PY - 1990/1/1
Y1 - 1990/1/1
N2 - The Transfusion Safety Study (TSS) is a multicenter, cooperative investigation of factors that may determine the occurrence and modify the expression of transusion-transmitted infections. A flow cytometry laboratory was established in each of the six participating centers in order to avoid alterations in cell phenotypes which may be caused by shipping delays, temperature changes, and handling. As a consequence, in order to assure compatibility of results, stringent standardization, quality control, and proficiency testing procedures were developed. This paper documents (i) the effect of time from phlebotomy to specimen staining and then to analysis for the antibodies used in the study; (ii) the effects of variations in light scatter cursor location for certain antibodies; (iii) a quality control program and data management and analysis system, each specifically designed for the study; and (iv) presents extensive data on age- and sex-related reference (normal) ranges for the several individual and paired monoclonal antibodies used in the study. Problems encountered, including obtaining reliable absolute lymphocyte counts, interference by nucleated erythrocytes, and sources of variability in results, are discussed. This study is meant to serve as a reference for future TSS publications.
AB - The Transfusion Safety Study (TSS) is a multicenter, cooperative investigation of factors that may determine the occurrence and modify the expression of transusion-transmitted infections. A flow cytometry laboratory was established in each of the six participating centers in order to avoid alterations in cell phenotypes which may be caused by shipping delays, temperature changes, and handling. As a consequence, in order to assure compatibility of results, stringent standardization, quality control, and proficiency testing procedures were developed. This paper documents (i) the effect of time from phlebotomy to specimen staining and then to analysis for the antibodies used in the study; (ii) the effects of variations in light scatter cursor location for certain antibodies; (iii) a quality control program and data management and analysis system, each specifically designed for the study; and (iv) presents extensive data on age- and sex-related reference (normal) ranges for the several individual and paired monoclonal antibodies used in the study. Problems encountered, including obtaining reliable absolute lymphocyte counts, interference by nucleated erythrocytes, and sources of variability in results, are discussed. This study is meant to serve as a reference for future TSS publications.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025215920&partnerID=8YFLogxK
U2 - 10.1016/0090-1229(90)90097-A
DO - 10.1016/0090-1229(90)90097-A
M3 - Article
C2 - 2182228
AN - SCOPUS:0025215920
VL - 55
SP - 187
EP - 220
JO - Clinical Immunology
JF - Clinical Immunology
SN - 1521-6616
IS - 2
ER -