Leukocyte immunophenotyping by flow cytometry in a multisite study: Standardization, quality control, and normal values in the Transfusion Safety Study

John W. Parker, Bernard Adelsberg, Stanley P. Azen, Donna Boone, Mary Ann Fletcher, George F. Gjerset, Joseph Hassett, Joseph Kaplan, Joyce C. Niland, Tamara Odom-Maryon, Eva A. Operskalski, Harry Prince, Diane Scott, Daniel P. Stites, James W. Mosley

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

The Transfusion Safety Study (TSS) is a multicenter, cooperative investigation of factors that may determine the occurrence and modify the expression of transusion-transmitted infections. A flow cytometry laboratory was established in each of the six participating centers in order to avoid alterations in cell phenotypes which may be caused by shipping delays, temperature changes, and handling. As a consequence, in order to assure compatibility of results, stringent standardization, quality control, and proficiency testing procedures were developed. This paper documents (i) the effect of time from phlebotomy to specimen staining and then to analysis for the antibodies used in the study; (ii) the effects of variations in light scatter cursor location for certain antibodies; (iii) a quality control program and data management and analysis system, each specifically designed for the study; and (iv) presents extensive data on age- and sex-related reference (normal) ranges for the several individual and paired monoclonal antibodies used in the study. Problems encountered, including obtaining reliable absolute lymphocyte counts, interference by nucleated erythrocytes, and sources of variability in results, are discussed. This study is meant to serve as a reference for future TSS publications.

Original languageEnglish
Pages (from-to)187-220
Number of pages34
JournalClinical Immunology and Immunopathology
Volume55
Issue number2
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

Fingerprint

Immunophenotyping
Quality Control
Flow Cytometry
Reference Values
Leukocytes
Safety
Erythroblasts
Phlebotomy
Antibodies
Lymphocyte Count
Publications
Monoclonal Antibodies
Staining and Labeling
Phenotype
Light
Temperature
Infection

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

Leukocyte immunophenotyping by flow cytometry in a multisite study : Standardization, quality control, and normal values in the Transfusion Safety Study. / Parker, John W.; Adelsberg, Bernard; Azen, Stanley P.; Boone, Donna; Fletcher, Mary Ann; Gjerset, George F.; Hassett, Joseph; Kaplan, Joseph; Niland, Joyce C.; Odom-Maryon, Tamara; Operskalski, Eva A.; Prince, Harry; Scott, Diane; Stites, Daniel P.; Mosley, James W.

In: Clinical Immunology and Immunopathology, Vol. 55, No. 2, 01.01.1990, p. 187-220.

Research output: Contribution to journalArticle

Parker, JW, Adelsberg, B, Azen, SP, Boone, D, Fletcher, MA, Gjerset, GF, Hassett, J, Kaplan, J, Niland, JC, Odom-Maryon, T, Operskalski, EA, Prince, H, Scott, D, Stites, DP & Mosley, JW 1990, 'Leukocyte immunophenotyping by flow cytometry in a multisite study: Standardization, quality control, and normal values in the Transfusion Safety Study', Clinical Immunology and Immunopathology, vol. 55, no. 2, pp. 187-220. https://doi.org/10.1016/0090-1229(90)90097-A
Parker, John W. ; Adelsberg, Bernard ; Azen, Stanley P. ; Boone, Donna ; Fletcher, Mary Ann ; Gjerset, George F. ; Hassett, Joseph ; Kaplan, Joseph ; Niland, Joyce C. ; Odom-Maryon, Tamara ; Operskalski, Eva A. ; Prince, Harry ; Scott, Diane ; Stites, Daniel P. ; Mosley, James W. / Leukocyte immunophenotyping by flow cytometry in a multisite study : Standardization, quality control, and normal values in the Transfusion Safety Study. In: Clinical Immunology and Immunopathology. 1990 ; Vol. 55, No. 2. pp. 187-220.
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