Leukemia translocation gene, PLZF, is expressed with a speckled nuclear pattern in early hematopoietic progenitors

A. Reid, A. Gould, N. Brand, M. Cook, P. Strutt, J. Li, J. Licht, S. Waxman, R. Krumlauf, Arthur Z Zelent

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

The PLZF gene was discovered by studying a rearrangement of the RARα locus in a patient with acute promyelocytic leukemia and a t(11;17) chromosomal translocation. To understand further the potential role(s) of the PLZF gene product in hematopoiesis, we have examined its expression levels in a variety of murine tissues and in established cell lines that are representative of various stages of myeloid and lymphoid development. We show that murine PLZF (mPLZF) is expressed at the highest levels in undifferentiated, multipotential hematopoietic progenitor cells and that its expression declines as cells become more mature and committed to various hematopoietic lineages. Data obtained with established cell lines are corroborated by results showing the lack of human PLZF protein expression in mature peripheral blood mononuclear cells and high PLZF levels in the nuclei of CD34+ human bone marrow progenitor cells. Interestingly, unlike many transcription factors, PLZF protein in these cells possesses distinct punctate nuclear distribution, suggesting its compartmentalization in the nucleus. Taken together, our data suggest a role for PLZF protein in early hematopoiesis and the requirement of downregulation of its expression for proper differentiation of most hematopoietic lineages.

Original languageEnglish (US)
Pages (from-to)4544-4552
Number of pages9
JournalBlood
Volume86
Issue number12
StatePublished - 1995
Externally publishedYes

Fingerprint

Hematopoiesis
Leukemia
Genes
Cells
Cell Line
Genetic Translocation
Acute Promyelocytic Leukemia
Hematopoietic Stem Cells
Bone Marrow Cells
Blood Cells
Bone
Proteins
Blood
Transcription Factors
Stem Cells
Down-Regulation
Tissue
human ZBTB16 protein

ASJC Scopus subject areas

  • Hematology

Cite this

Reid, A., Gould, A., Brand, N., Cook, M., Strutt, P., Li, J., ... Zelent, A. Z. (1995). Leukemia translocation gene, PLZF, is expressed with a speckled nuclear pattern in early hematopoietic progenitors. Blood, 86(12), 4544-4552.

Leukemia translocation gene, PLZF, is expressed with a speckled nuclear pattern in early hematopoietic progenitors. / Reid, A.; Gould, A.; Brand, N.; Cook, M.; Strutt, P.; Li, J.; Licht, J.; Waxman, S.; Krumlauf, R.; Zelent, Arthur Z.

In: Blood, Vol. 86, No. 12, 1995, p. 4544-4552.

Research output: Contribution to journalArticle

Reid, A, Gould, A, Brand, N, Cook, M, Strutt, P, Li, J, Licht, J, Waxman, S, Krumlauf, R & Zelent, AZ 1995, 'Leukemia translocation gene, PLZF, is expressed with a speckled nuclear pattern in early hematopoietic progenitors', Blood, vol. 86, no. 12, pp. 4544-4552.
Reid A, Gould A, Brand N, Cook M, Strutt P, Li J et al. Leukemia translocation gene, PLZF, is expressed with a speckled nuclear pattern in early hematopoietic progenitors. Blood. 1995;86(12):4544-4552.
Reid, A. ; Gould, A. ; Brand, N. ; Cook, M. ; Strutt, P. ; Li, J. ; Licht, J. ; Waxman, S. ; Krumlauf, R. ; Zelent, Arthur Z. / Leukemia translocation gene, PLZF, is expressed with a speckled nuclear pattern in early hematopoietic progenitors. In: Blood. 1995 ; Vol. 86, No. 12. pp. 4544-4552.
@article{d12ff4acfe0947aaa24be12c3139f330,
title = "Leukemia translocation gene, PLZF, is expressed with a speckled nuclear pattern in early hematopoietic progenitors",
abstract = "The PLZF gene was discovered by studying a rearrangement of the RARα locus in a patient with acute promyelocytic leukemia and a t(11;17) chromosomal translocation. To understand further the potential role(s) of the PLZF gene product in hematopoiesis, we have examined its expression levels in a variety of murine tissues and in established cell lines that are representative of various stages of myeloid and lymphoid development. We show that murine PLZF (mPLZF) is expressed at the highest levels in undifferentiated, multipotential hematopoietic progenitor cells and that its expression declines as cells become more mature and committed to various hematopoietic lineages. Data obtained with established cell lines are corroborated by results showing the lack of human PLZF protein expression in mature peripheral blood mononuclear cells and high PLZF levels in the nuclei of CD34+ human bone marrow progenitor cells. Interestingly, unlike many transcription factors, PLZF protein in these cells possesses distinct punctate nuclear distribution, suggesting its compartmentalization in the nucleus. Taken together, our data suggest a role for PLZF protein in early hematopoiesis and the requirement of downregulation of its expression for proper differentiation of most hematopoietic lineages.",
author = "A. Reid and A. Gould and N. Brand and M. Cook and P. Strutt and J. Li and J. Licht and S. Waxman and R. Krumlauf and Zelent, {Arthur Z}",
year = "1995",
language = "English (US)",
volume = "86",
pages = "4544--4552",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "12",

}

TY - JOUR

T1 - Leukemia translocation gene, PLZF, is expressed with a speckled nuclear pattern in early hematopoietic progenitors

AU - Reid, A.

AU - Gould, A.

AU - Brand, N.

AU - Cook, M.

AU - Strutt, P.

AU - Li, J.

AU - Licht, J.

AU - Waxman, S.

AU - Krumlauf, R.

AU - Zelent, Arthur Z

PY - 1995

Y1 - 1995

N2 - The PLZF gene was discovered by studying a rearrangement of the RARα locus in a patient with acute promyelocytic leukemia and a t(11;17) chromosomal translocation. To understand further the potential role(s) of the PLZF gene product in hematopoiesis, we have examined its expression levels in a variety of murine tissues and in established cell lines that are representative of various stages of myeloid and lymphoid development. We show that murine PLZF (mPLZF) is expressed at the highest levels in undifferentiated, multipotential hematopoietic progenitor cells and that its expression declines as cells become more mature and committed to various hematopoietic lineages. Data obtained with established cell lines are corroborated by results showing the lack of human PLZF protein expression in mature peripheral blood mononuclear cells and high PLZF levels in the nuclei of CD34+ human bone marrow progenitor cells. Interestingly, unlike many transcription factors, PLZF protein in these cells possesses distinct punctate nuclear distribution, suggesting its compartmentalization in the nucleus. Taken together, our data suggest a role for PLZF protein in early hematopoiesis and the requirement of downregulation of its expression for proper differentiation of most hematopoietic lineages.

AB - The PLZF gene was discovered by studying a rearrangement of the RARα locus in a patient with acute promyelocytic leukemia and a t(11;17) chromosomal translocation. To understand further the potential role(s) of the PLZF gene product in hematopoiesis, we have examined its expression levels in a variety of murine tissues and in established cell lines that are representative of various stages of myeloid and lymphoid development. We show that murine PLZF (mPLZF) is expressed at the highest levels in undifferentiated, multipotential hematopoietic progenitor cells and that its expression declines as cells become more mature and committed to various hematopoietic lineages. Data obtained with established cell lines are corroborated by results showing the lack of human PLZF protein expression in mature peripheral blood mononuclear cells and high PLZF levels in the nuclei of CD34+ human bone marrow progenitor cells. Interestingly, unlike many transcription factors, PLZF protein in these cells possesses distinct punctate nuclear distribution, suggesting its compartmentalization in the nucleus. Taken together, our data suggest a role for PLZF protein in early hematopoiesis and the requirement of downregulation of its expression for proper differentiation of most hematopoietic lineages.

UR - http://www.scopus.com/inward/record.url?scp=0028818983&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028818983&partnerID=8YFLogxK

M3 - Article

C2 - 8541544

AN - SCOPUS:0028818983

VL - 86

SP - 4544

EP - 4552

JO - Blood

JF - Blood

SN - 0006-4971

IS - 12

ER -